Majada J P, Sierra M I, Sánchez-Tamés R
Laboratory of Plant Physiology, Department BOS, University of Oviedo, Catedrático Rodrigo Uría s/n 33071 Oviedo, Spain, , , , , , ES.
Plant Cell Rep. 2000 Jul;19(8):825-830. doi: 10.1007/s002990000196.
We have developed a high-yielding procedure for the in vitro propagation of juvenile material of Taxus baccata involving a combination of seed handling and culture on WP culture medium supplemented with sucrose (2%), activated charcoal (0.5%) and BAP (22.19 mM) for 30 days, followed by 40 days on hormone-free medium. Shoot apical ends should be decapitated to obtain propagation rates up to 12- to 18-fold per subculture period (70 days). In this way the high genetic variability of the juvenile material can be used in the most productive way. In addition to producing large numbers of yew plants (difficult to get by traditional methods), this procedure allows the fast screening of individuals for their taxane content. A negative correlation between growth and secondary metabolite content was found for paclitaxel. The positive correlation with 10-deacetyl baccatin III accumulation reflects once more the commercial viability of using 10-deacetyl baccatin III extraction as an alternative to taxane production, but this time opening up the possibility of selecting genotypes with both characteristics: fast growth and high productivity.
我们已经开发出一种高产程序，用于欧洲红豆杉幼嫩材料的离体繁殖，该程序包括种子处理以及在添加了蔗糖（2%）、活性炭（0.5%）和BAP（22.19 mM）的WP培养基上培养30天，随后在无激素培养基上培养40天。茎尖应进行摘心处理，以在每个继代培养周期（70天）获得高达12至18倍的繁殖率。通过这种方式，可以以最高效的方式利用幼嫩材料的高遗传变异性。除了能大量生产紫杉植物（这是传统方法难以实现的）外，该程序还能快速筛选个体的紫杉烷含量。对于紫杉醇，发现生长与次生代谢物含量之间存在负相关。与10-去乙酰巴卡亭III积累的正相关再次反映了使用10-去乙酰巴卡亭III提取作为紫杉烷生产替代方法的商业可行性，但这次开辟了选择具有快速生长和高生产力这两种特性的基因型的可能性。
