通过体细胞胚胎发生实现冰花高效植株再生。

Efficient plant regeneration of Mesembryanthemum crystallinum via somatic embryogenesis.

作者信息

Cushman J C, Wulan T, Kuscuoglu N, Spatz M D

机构信息

Department of Biochemistry and Molecular Biology, 147 Noble Research Center, Oklahoma State University, Stillwater, Oklahoma, USA Fax: +1-405-744-7799 e-mail:

Department of Biochemistry, 540 Biosciences West, University of Arizona, Tucson, AZ 85721 USA, , , , , , US.

出版信息

Plant Cell Rep. 2000 Apr;19(5):459-463. doi: 10.1007/s002990050756.

DOI:10.1007/s002990050756

PMID:30754883

Abstract

An efficient plant regeneration procedure has been established from hypocotyl explants of the common ice plant, Mesembryanthemum crystallinum L, a halophytic leaf succulent that exhibits a stress-induced switch from C3 photosynthesis to crassulacean acid metabolism (CAM). Somatic embryos were initiated and developed up to globular and heart stages in Murashige and Skoog (MS) media supplemented with 3% sucrose, 0.6% bacto-agar, 80 mM NaCl, 5 μM 2,4-D and 1 μM kinetin. High frequency regeneration occurred when somatic embryos were germinated on media that lacked 2,4-D. High cytokinin treatment suppressed normal growth of embryos and favored abnormal embryo proliferation. Without growth regulators, regenerated plants rooted on MS medium with 100% efficiency. Mature, regenerated plants were fertile and morphologically identical to seed-derived plants.

摘要

已从盐生肉质叶植物——冰叶日中花（Mesembryanthemum crystallinum L）的下胚轴外植体建立了一种高效的植株再生程序，该植物在胁迫诱导下会从C3光合作用转变为景天酸代谢（CAM）。在添加了3%蔗糖、0.6%细菌琼脂、80 mM氯化钠、5 μM 2,4 - D和1 μM激动素的Murashige和Skoog（MS）培养基中，体细胞胚开始形成并发育至球形期和心形期。当体细胞胚在不含2,4 - D的培养基上萌发时，发生了高频再生。高细胞分裂素处理抑制了胚的正常生长并促进了异常胚的增殖。在没有生长调节剂的情况下，再生植株在MS培养基上生根效率达100%。成熟的再生植株可育，且形态上与种子衍生的植株相同。