Pinheiro A A, Pozzobon M T, do Valle C B, Penteado M I O, Carneiro V T C
Centro Nacional de Pesquisa em Recursos Genéticos e Biotecnologia, CENARGEN/EMBRAPA, SAIN Parque Rural, Brasília, DF, Brazil, , , , , , BR.
Centro Nacional de Pesquisa de Gado de Corte, CNPGC/DMBRAPA, Campo Grande, MS, Brazil, , , , , , BR.
Plant Cell Rep. 2000 Jan;19(3):274-278. doi: 10.1007/s002990050011.
Some species of Brachiaria, generally tetraploid apomictic varieties, have become important forage grasses in the tropics. Breeding of Brachiaria depends on compatibility with the available apomitic tretraploid cultivars. This paper describes a procedure for chromosome duplication of two Bracharia brizantha diploid sexual accessions, using colchicine treatment of basal segments of in-vitro-grown plants. Explants were cultured on a medium containing 1 mg/l naphthaleneacetic acid, 3 mg/l kinetin and 0.01% colchicine for 48 h and transferred to the same medium without colchicine until shoot regeneration occurred. Regenerated plants were screened by flow cytometry, and chromosome number duplication was confirmed by cytological analysis of root tips.
臂形草属的一些物种，通常是四倍体无融合生殖品种，已成为热带地区重要的饲用禾本科植物。臂形草的育种依赖于与现有的无融合生殖四倍体栽培品种的亲和性。本文描述了一种对两株二倍体有性生殖的臂形草进行染色体加倍的方法，即使用秋水仙素处理离体培养植株的基部切段。外植体在含有1毫克/升萘乙酸、3毫克/升激动素和0.01%秋水仙素的培养基上培养48小时，然后转移到不含秋水仙素的相同培养基上，直至再生出芽。通过流式细胞术对再生植株进行筛选，并通过根尖细胞学分析确认染色体数目加倍。
