Kobayashi S, Ding C K, Nakamura Y, Nakajima I, Matsumoto R
Persimmon and Grape Research Center, National Institute of Fruit Tree Science, Akitsu, Hiroshima 729-2494, Japan Fax: +81-846-45-5370 e-mail:
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China, , , , , , CN.
Plant Cell Rep. 2000 Sep;19(9):904-910. doi: 10.1007/s002990000203.
A stilbene synthase gene was isolated from three Vitis spp. (V. vinifera, V. labrusca and V. riparia). These genes were placed under the control of the cauliflower mosaic virus 35S promoter and introduced into kiwifruit (Actinidia deliciosa) plants by Agrobacterium-mediated gene transfer. The introduced gene(s) were expressed and piceid (resveratrol-glucoside) rather than resveratrol was produced in the leaves of the transformants. Resveratrol produced by the action of the integrated gene(s) seems to be metabolized into piceid by an endogenous glycosyltransferase. Among the transformants obtained, the highest piceid content in the young leaves was 182 μg/g fresh weight. Although these transformants did not show resistance against Botrytis cinerea, which causes gray-mould disease, the fruits may have some beneficial effects on human health.
从三种葡萄属植物（欧亚葡萄、美洲葡萄和河岸葡萄）中分离出一个芪合酶基因。这些基因置于花椰菜花叶病毒35S启动子的控制之下，并通过农杆菌介导的基因转移导入猕猴桃（美味猕猴桃）植株。导入的基因得以表达，转基因植株叶片中产生的是白藜芦醇苷（白藜芦醇 - 葡萄糖苷）而非白藜芦醇。整合基因作用产生的白藜芦醇似乎被一种内源性糖基转移酶代谢为白藜芦醇苷。在所获得的转基因植株中，幼叶中白藜芦醇苷的最高含量为182μg/g鲜重。尽管这些转基因植株对引起灰霉病的灰葡萄孢没有抗性，但这些果实可能对人体健康有一些有益作用。
