Yao Wantian, Kong Lingling, Lei Diya, Zhao Bing, Tang Honglan, Zhou Xuan, Lin Yuanxiu, Zhang Yunting, Wang Yan, He Wen, Li Mengyao, Chen Qing, Luo Ya, Wang Xiaorong, Tang Haoru, Zhang Yong
College of Horticulture, Sichuan Agricultural University, Chengdu, China.
Front Plant Sci. 2023 Jul 31;14:1204267. doi: 10.3389/fpls.2023.1204267. eCollection 2023.
The all-red () is an anthocyanin-rich and excellent hardy fruit. Many studies have focused on the green-fleshed , and fewer studies have been conducted on the all-red . Here we reported a regeneration and Agrobacterium-mediated transformation protocol by using leaves of all-red as explants. Aseptic seedling leaves of were used as callus-inducing materials. MS medium supplemented with 0.3 mg·L 2,4-D and 1.0 mg·L BA was the optimal medium for callus induction of leaves, and medium supplemented with 3 mg·L tZ and 0.5 mg·L IAA was optimal for adventitious shoot regeneration. The best proliferation medium for adventitious buds was MS + 1.0 mg·L BA + 0.3 mg·L NAA. The best rooting medium was 1/2MS + 0.7 mg·L IBA with a 100% rooting rate. For the red flesh hardy kiwi variety 'Purpurna Saduwa' ( var. ), leaves are receptors for Agrobacterium (EHA105)-mediated transformation. The orthogonal experiment was used for the optimization of each genetic transformation parameter and the genetic transformation of the leaves was 21% under optimal conditions. Our study provides technical parameters for applying genetic resources and molecular breeding of kiwifruit with red flesh.
全红（品种名）是一种富含花青素且抗逆性强的优良果实。许多研究聚焦于绿肉（品种名），而对全红（品种名）的研究较少。在此，我们报道了一种以全红（品种名）叶片为外植体的再生及农杆菌介导的转化方案。（品种名）无菌幼苗叶片用作愈伤组织诱导材料。添加0.3 mg·L 2,4 - D和1.0 mg·L BA的MS培养基是叶片愈伤组织诱导的最佳培养基，添加3 mg·L tZ和0.5 mg·L IAA的培养基对不定芽再生最佳。不定芽最佳增殖培养基为MS + 1.0 mg·L BA + 0.3 mg·L NAA。最佳生根培养基为1/2MS + 0.7 mg·L IBA，生根率达100%。对于红肉抗逆猕猴桃品种‘Purpurna Saduwa’（品种名），叶片是农杆菌（EHA105）介导转化的受体。采用正交试验优化各遗传转化参数，在最佳条件下叶片的遗传转化率为21%。我们的研究为红肉猕猴桃遗传资源利用和分子育种提供了技术参数。
