Dolfi A, Bianchi F, Lupetti M
Istituto Anatomico, Università di Pisa, Italy.
J Anat. 1988 Oct;160:201-10.
The present study was carried out on the Diffusely Infiltrated Area of the bursa of Fabricius (DIA) and the area of lymphoid infiltration of the dorsal wall of the cloaca, using immunoperoxidase and electron transmission microscopy, in order to verify the behaviour of antibody-producing cells and their distribution in the two organs. An anti-chicken Ig antiserum was used for this purpose, and the results obtained show that there is a progressive increase in the antibody-producing cells in the DIA, and subsequently also in the cloaca, during the first few weeks of life; the percentage values remain constantly higher in the DIA compared with the cloaca. Electron microscopy made it possible to detect numerous lymphocytes and some plasma cells in the epithelium. Many of the intra-epithelial lymphocytes were immunoperoxidase-positive. The possible role played by the B-lymphocytes found in both organs is discussed.
本研究利用免疫过氧化物酶和电子透射显微镜技术，对法氏囊的弥漫浸润区（DIA）和泄殖腔背壁的淋巴浸润区进行了研究，以验证抗体产生细胞的行为及其在这两个器官中的分布。为此使用了抗鸡Ig抗血清，所得结果表明，在生命的最初几周内，DIA中抗体产生细胞逐渐增加，随后泄殖腔中的抗体产生细胞也逐渐增加；与泄殖腔相比，DIA中的百分比值始终较高。电子显微镜能够检测到上皮中有大量淋巴细胞和一些浆细胞。许多上皮内淋巴细胞免疫过氧化物酶呈阳性。文中讨论了在这两个器官中发现的B淋巴细胞可能发挥的作用。
