Shin D M, Gupta V, Donner L, Chawla S, Benjamin R, Gutterman J, Blick M
Department of Medical Oncology, University of Texas M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Anticancer Res. 1987 Nov-Dec;7(6):1117-23.
Protooncogenes have the ability to induce and/or maintain the transformed state when they are overly expressed or altered by mutation or gene rearrangement. To study the possible involvement of these cellular oncogenes in the neoplastic transformation, we have analyzed their expression in 44 fresh samples obtained from primary, recurrent, or metastatic tumors from patients with a variety of sarcomas and a melanoma. Our analysis was carried out by the northern blot technique using poly (A)+ RNA hybridized with human cellular DNA probes. A normal 2.3-kb c-myc transcript was observed almost universally at various levels. A normal c-k-ras transcript of 5.2-kb was detected at a low level in most of the samples. In three samples we detected aberrant c-k-ras transcripts of 7.0 and 8.5-kb, while in two other samples we found an aberrant lower-molecular-weight transcript of 1.4-kb. N-myc was expressed in only three samples, and in all instances, the transcripts were aberrant (more than 10-kb). A normal 3.7-kb c-sis transcript was expressed at a low level in most of the sarcomas and the melanoma but was uniquely overexpressed in giant cell tumors of bone. C-fos (2.2-kb) was expressed at a low level in almost half of the samples; c-myb was never expressed. We conclude that c-k-ras, n-myc, c-sis, and c-myc are aberrantly or overexpressed in sarcoma/melanoma, and their activation may play a role in the transforming events leading to development and/or progression of these tumors.
原癌基因在过度表达、因突变或基因重排而发生改变时，具有诱导和/或维持转化状态的能力。为了研究这些细胞癌基因在肿瘤转化中可能发挥的作用，我们分析了从患有各种肉瘤和黑色素瘤的患者的原发性、复发性或转移性肿瘤中获取的44份新鲜样本中它们的表达情况。我们的分析是通过Northern印迹技术进行的，使用与人类细胞DNA探针杂交的聚腺苷酸加尾（poly(A)+）RNA。几乎在所有样本中都普遍观察到正常的2.3kb c-myc转录本，不过表达水平各异。在大多数样本中检测到低水平的正常5.2kb c-k-ras转录本。在三个样本中，我们检测到7.0kb和8.5kb的异常c-k-ras转录本，而在另外两个样本中，我们发现了1.4kb的异常低分子量转录本。N-myc仅在三个样本中表达，并且在所有情况下，转录本都是异常的（超过10kb）。正常的3.7kb c-sis转录本在大多数肉瘤和黑色素瘤中低水平表达，但在骨巨细胞瘤中独特地过度表达。C-fos（2.2kb）在几乎一半的样本中低水平表达；c-myb从未表达。我们得出结论，c-k-ras、n-myc、c-sis和c-myc在肉瘤/黑色素瘤中异常表达或过度表达，它们的激活可能在导致这些肿瘤发生和/或进展的转化事件中起作用。
