BIOCEV, First Faculty of Medicine, Charles University, Průmyslová 595, 252 50 Vestec, Czech Republic.
Chem Commun (Camb). 2019 Feb 26;55(18):2696-2699. doi: 10.1039/c8cc09638d.
Four novel fluorescent cores bearing a transformable functional group based on a π-expanded naphthalimide including a fused pyranone or furan ring have been prepared. Fluorescent probes LysoSers 13-16 for lysosomal targeting have been tested. Co-localization with a commercial lysosome specific marker confirmed that the LysoSers labeled the lysosomal compartment with high selectivity. The LysoSers show excellent brightness and low toxicity.
已经制备了四个新型荧光核心，它们基于一个可变形的官能团，该官能团基于一个扩展的萘酰亚胺，包括一个融合的吡喃酮或呋喃环。已经测试了用于溶酶体靶向的荧光探针 LysoSers 13-16。与商业溶酶体特异性标记物的共定位证实，LysoSers 标记溶酶体具有高选择性。LysoSers 具有出色的亮度和低毒性。
