Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, 210095, China.
Curr Microbiol. 2019 Apr;76(4):435-441. doi: 10.1007/s00284-019-01644-5.
The effects of nitroglycerine (NG) on the rumen methane emission, fermentation, and microbial community of Hu sheep were investigated. Eight sheep were fed NG (100 mg/head/day); another eight sheep served as controls. NG decreased methane emission of Hu sheep by ~ 19.3% (P < 0.05) without adversely affecting the production performance or rumen fermentation (P > 0.05). The alpha and beta diversity indexes of the bacterial and archaeal community showed no significant differences (P > 0.05). The dominant methanogenic species was the Methanobrevibacter gottschalkii clade, accounting for ~ 60%, followed by the Methanobrevibacter boviskoreani and Methanobrevibacter ruminantium clades. Prevotella 1 was the most dominant bacterial genus, accounting for ~ 42%, followed by the Rikenellaceae RC9 and Bacteroidales BS11 gut groups. In addition, pearson correlation analysis showed a few Methanomassiliicoccales species significantly correlated with several bacterial genera (P < 0.05).
研究了硝酸甘油（NG）对湖羊瘤胃甲烷排放、发酵和微生物群落的影响。给 8 只羊喂食 NG（100mg/头/天）；另外 8 只羊作为对照。NG 降低了湖羊的甲烷排放量约 19.3%（P<0.05），但对生产性能或瘤胃发酵没有不利影响（P>0.05）。细菌和古菌群落的 alpha 和 beta 多样性指数没有显著差异（P>0.05）。主要的产甲烷物种是 Methanobrevibacter gottschalkii 进化枝，占60%，其次是 Methanobrevibacter boviskoreani 和 Methanobrevibacter ruminantium 进化枝。普雷沃氏菌 1 是最主要的细菌属，占42%，其次是 Rikenellaceae RC9 和 Bacteroidales BS11 肠道群。此外，皮尔逊相关分析显示，少数 Methanomassiliicoccales 物种与几个细菌属显著相关（P<0.05）。
