Li Jichao, Zhao Shengwei, Meng Zhenxiang, Gao Yunlong, Miao Jing, Mao Shengyong, Jin Wei
Ruminant Nutrition and Feed Engineering Technology Research Center, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Biology (Basel). 2023 Jul 15;12(7):1011. doi: 10.3390/biology12071011.
This study aimed to investigate the effects of fumarate and nitroglycerin on rumen fermentation, methane and hydrogen production, and microbiota. In vitro rumen fermentation was used in this study with four treatment groups: control (CON), fumarate (FA), nitroglycerin (NG) and fumarate plus nitroglycerin (FN). Real-time PCR and 16S rRNA gene sequencing were used to analyze microbiota. The results showed that nitroglycerin completely inhibited methane production and that this resulted in hydrogen accumulation. Fumarate decreased the hydrogen accumulation and improved the rumen fermentation parameters. Fumarate increased the concentration of propionate and microbial crude protein, and decreased the ratio of acetate to propionate in FN. Fumarate, nitroglycerin and their combination did not affect the abundance of bacteria, protozoa and anaerobic fungi, but altered archaea. The PCoA showed that the bacterial (Anosim, R = 0.747, = 0.001) and archaeal communities (Anosim, R = 0.410, = 0.005) were different among the four treatments. Compared with CON, fumarate restored Bacteroidetes, Firmicutes, Spirochaetae, Actinobacteria, Unclassified , , and in relative abundance in FN, but did not affect , and archaeal taxa. The results indicated that fumarate alleviated the depressed rumen fermentation caused by the inhibition of methanogenesis by nitroglycerin. This may potentially provide an alternative way to use these chemicals to mitigate methane emission in ruminants.
本研究旨在探讨富马酸盐和硝酸甘油对瘤胃发酵、甲烷和氢气产生以及微生物群的影响。本研究采用体外瘤胃发酵，设置四个处理组：对照组（CON）、富马酸盐组（FA）、硝酸甘油组（NG）和富马酸盐加硝酸甘油组（FN）。采用实时荧光定量PCR和16S rRNA基因测序分析微生物群。结果表明，硝酸甘油完全抑制了甲烷的产生，并导致氢气积累。富马酸盐减少了氢气积累，改善了瘤胃发酵参数。富马酸盐提高了丙酸浓度和微生物粗蛋白含量，并降低了FN组中乙酸与丙酸的比例。富马酸盐、硝酸甘油及其组合对细菌、原生动物和厌氧真菌的丰度没有影响，但改变了古菌的丰度。主坐标分析显示，四种处理之间的细菌群落（Anosim，R = 0.747，P = 0.001）和古菌群落（Anosim，R = 0.410，P = 0.005）存在差异。与CON组相比，富马酸盐恢复了FN组中拟杆菌门、厚壁菌门、螺旋体门、放线菌门、未分类的、、和的相对丰度，但对、和古菌类群没有影响。结果表明，富马酸盐减轻了硝酸甘油抑制甲烷生成所导致的瘤胃发酵抑制。这可能为利用这些化学物质减少反刍动物甲烷排放提供一种替代方法。
