Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Biol Trace Elem Res. 2019 Dec;192(2):196-205. doi: 10.1007/s12011-019-1647-4. Epub 2019 Feb 12.
Selenium (Se) is an essential micronutrient that maintains normal physiological functions in humans and animals. Se plays a vital role in regulating smooth muscle contractions, and selenoprotein N (SelN), selenoprotein T (SelT), and selenoprotein W (SelW) are closely related to the release of Ca. The present study analyzed the effects and mechanisms of SelN, SelT, and SelW in uterine smooth muscle contractions in a mouse model fed Se. The mRNA and protein levels in the uterine smooth muscle of mice were detected by qPCR, Western blot, and immunohistochemical analysis. The results showed that Se played an indispensable role in uterine smooth muscle contractions. Increased Se concentration in food increased the release of Ca to a certain extent, causing CaM expression, MLCK expression, and MLC phosphorylation, which can lead to uterine smooth muscle contractions. In contrast, Se deficiency reduced the release of Ca to a certain degree, thereby reducing the contractile ability of uterine smooth muscle. In this study, genes related to SelN, SelT, and SelW expression in uterine smooth muscle cells were investigated. The results showed that the Se concentration had an effect on the expression of SelN, SelT, and SelW in uterine smooth muscle cells. Se influences the release of Ca through SelN, SelT, and SelW, which changes the expression of MLCK and then affects uterine smooth muscle contractions. The three selenoproteins SelN, SelT, and SelW play a very important role in uterine smooth muscle contractions, and the absence of any of these proteins affects the contractility of the uterus.
硒（Se）是一种必需的微量元素，它维持着人类和动物的正常生理功能。Se 在调节平滑肌收缩中起着至关重要的作用，而硒蛋白 N（SelN）、硒蛋白 T（SelT）和硒蛋白 W（SelW）与 Ca 的释放密切相关。本研究分析了 Se 喂养的小鼠模型中 SelN、SelT 和 SelW 对子宫平滑肌收缩的影响及其机制。通过 qPCR、Western blot 和免疫组织化学分析检测小鼠子宫平滑肌中的 mRNA 和蛋白水平。结果表明，Se 在子宫平滑肌收缩中起着不可或缺的作用。食物中 Se 浓度的增加在一定程度上增加了 Ca 的释放，导致 CaM 表达、MLCK 表达和 MLC 磷酸化，从而导致子宫平滑肌收缩。相反，Se 缺乏在一定程度上减少了 Ca 的释放，从而降低了子宫平滑肌的收缩能力。在这项研究中，研究了与 SelN、SelT 和 SelW 在子宫平滑肌细胞中表达相关的基因。结果表明，Se 浓度对子宫平滑肌细胞中 SelN、SelT 和 SelW 的表达有影响。Se 通过 SelN、SelT 和 SelW 影响 Ca 的释放，改变 MLCK 的表达，从而影响子宫平滑肌收缩。这三种硒蛋白 SelN、SelT 和 SelW 在子宫平滑肌收缩中起着非常重要的作用，其中任何一种蛋白的缺失都会影响子宫的收缩能力。
