Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Biol Trace Elem Res. 2018 May;183(1):138-146. doi: 10.1007/s12011-017-1130-z. Epub 2017 Aug 23.
Selenium (Se) is an essential micronutrient affecting various aspects of health. The balance of the Se concentration has an important protective and promoter effect on physiological function in inducing muscular disorders in smooth muscle. Selenoprotein N (SelN) is closely related to Ca release. The present study aimed to determine the effects and mechanism of action of dietary Se on uterine smooth muscle contraction via SelN using a mouse model. Quantitative polymerase chain reaction (qPCR) analysis was performed to detect mRNA levels. Western blotting was performed to detect protein levels. The results of the immunohistochemical analysis showed that Se had an effect on the uterine smooth muscle. The Se-supplement increased the release of Ca, Ca-calmodulin (CaM) expression, myosin light chain kinase (MLCK) expression, and myosin light chain (MLC) phosphorylation but did not affect ROCK and RhoA in uterine smooth muscle. Furthermore, the lack of Se showed an opposite impact. The effects of Se regulation were closely related to SelN. The interference of mouse SelN was performed on the uterine smooth muscle cell. Additionally, the results displayed the regulation of Se on the release of Ca, CaM expression, MLCK expression, and MLC phosphorylation were significant inhibited, and there was no effect on ROCK and RhoA. In conclusion, Se played an important role in regulating the process of contraction in uterine smooth muscle with SelN.
硒（Se）是一种影响健康各个方面的必需微量元素。硒浓度的平衡对平滑肌诱导肌肉紊乱的生理功能具有重要的保护和促进作用。硒蛋白 N（SelN）与 Ca 释放密切相关。本研究旨在通过小鼠模型确定膳食 Se 通过 SelN 对子宫平滑肌收缩的影响及其作用机制。采用定量聚合酶链反应（qPCR）分析检测 mRNA 水平，采用 Western blot 检测蛋白水平。免疫组织化学分析结果表明，Se 对子宫平滑肌有作用。补充 Se 增加 Ca 的释放、Ca-钙调蛋白（CaM）表达、肌球蛋白轻链激酶（MLCK）表达和肌球蛋白轻链（MLC）磷酸化，但不影响子宫平滑肌中的 ROCK 和 RhoA。此外，缺乏 Se 则表现出相反的影响。Se 的调节作用与 SelN 密切相关。对子宫平滑肌细胞进行小鼠 SelN 干扰，结果显示 Se 对 Ca 释放、CaM 表达、MLCK 表达和 MLC 磷酸化的调节作用显著受到抑制，对 ROCK 和 RhoA 没有影响。综上所述，Se 通过 SelN 在调节子宫平滑肌收缩过程中发挥重要作用。
