Diaz-Espada F, Martinez-Alonso C, Bernabe R R
J Immunol. 1978 Jul;121(1):13-8.
Mice were injected with DNP-derivatives of thymus dependent (DNP-OA) and thymus-independent (DNP-Ficoll and TNP-LPS) antigens, and the response to TNP-LPS in vitro was studied after several priming periods. DNP-OA priming decreases the amount of cells responding to TNP-LPS in vitro. In the case of DNP-Ficoll and TNP-LPS-primed cells, there is an initial burst of responsiveness to TNP-LPS, which progressively decreases until an abolishment of TNP-LPS responsiveness is found at day 40 after immunization. The sensitivity to TNP-LPS reappears as new precursor cells differentiate into mature cells. We suggest that B cells progressively gain the capacity to respond to thymus-independent and thymus-dependent antigens (B1-B2 differentiation) and that challenge with a particle antigen increases the ratio of maturation throughout the pathway.
给小鼠注射胸腺依赖性（DNP-OA）和胸腺非依赖性（DNP-菲可和TNP-LPS）抗原的DNP衍生物，并在几个致敏期后研究体外对TNP-LPS的反应。DNP-OA致敏会减少体外对TNP-LPS作出反应的细胞数量。对于DNP-菲可和TNP-LPS致敏的细胞，最初对TNP-LPS有反应性爆发，随后逐渐降低，直到免疫后第40天发现TNP-LPS反应性完全消失。随着新的前体细胞分化为成熟细胞，对TNP-LPS的敏感性会再次出现。我们认为B细胞逐渐获得对胸腺非依赖性和胸腺依赖性抗原作出反应的能力（B1-B2分化），并且用颗粒抗原进行激发会增加整个途径中的成熟比例。
