Diaz-Espada F, Lopez-Alarcon L
Immunology. 1982 Aug;46(4):705-12.
Activated mouse spleen lymphocytes have increased amounts of the glycolytic enzymes pyruvate kinase and lactate dehydrogenase. No changes were found in hexokinase and in the gluconeogenic enzyme fructose 1,6-diphosphatase. Concanavalin A-activated T cells give higher activities of those enzymes than lipopolysaccharide-activated B lymphoblasts. Insulin treatment results in a stronger increment of the enzyme activity of mitogen-activated cells. Insulin inhibits the initial proliferation induced by either Con A or LPS, but a 50% increase in antibody-forming cells was found in LPS-treated cultures. Insulin may favour the differentiation of activated cells by increasing the rate of the glycolytic pathway.
活化的小鼠脾淋巴细胞中糖酵解酶丙酮酸激酶和乳酸脱氢酶的含量增加。己糖激酶和糖异生酶果糖1,6 -二磷酸酶未发现变化。伴刀豆球蛋白A活化的T细胞比脂多糖活化的B淋巴母细胞具有更高的这些酶活性。胰岛素处理导致有丝分裂原活化细胞的酶活性有更强的增加。胰岛素抑制由伴刀豆球蛋白A或脂多糖诱导的初始增殖，但在脂多糖处理的培养物中发现抗体形成细胞增加了50%。胰岛素可能通过提高糖酵解途径的速率促进活化细胞的分化。
