Bona C, Yano A, Dimitriu A, Miller R G
J Exp Med. 1978 Jul 1;148(1):136-47. doi: 10.1084/jem.148.1.136.
The B-cell mitogens lipopolysaccharide (LPS), Nocardia water-soluble mitogen (NWSM), and dextran sulfate (DxS) react with different subpopulations of B lymphocytes. Selective in vitro killing of cells responding to either LPS or NWSM has little effect on the in vitro response to the other mitogen, although the response to DxS is reduced in both cases. If, after selective in vitro killing, cells are injected into irradiated mice for 2-3 wk before measuring their in vitro mitogen responses, the same specificity pattern is seen. Thus, one is dealing with different B-cell subpopulations rather than different stages of maturation of a single population. Treatment with various alloantisera and complement before measuring the mitogen response to LPS and NWSM shows that (a) whereas all LPS response cells carry surface Ig, a subpopulation of NWSM responsive cells does not; (b) both LPS- and NWSM-responsive cells carry I-A antigens but might not I-E or I-J antigens; (c) all LPS-responsive cells carry I-C antigens, whereas approximately 25% of NWSM responsive cells do not: (d) there is a subpopulation of NWSM-responsive cells carrying neither surface Ig nor I-C antigens and resistant to anti-theta treatment.
B 细胞促有丝分裂原脂多糖(LPS)、诺卡氏菌水溶性促有丝分裂原(NWSM)和硫酸葡聚糖(DxS)与不同亚群的 B 淋巴细胞发生反应。对 LPS 或 NWSM 有反应的细胞进行体外选择性杀伤,对另一种促有丝分裂原的体外反应影响很小,不过在这两种情况下对 DxS 的反应都会降低。如果在体外选择性杀伤后,在测量细胞的体外促有丝分裂原反应之前,将细胞注射到经照射的小鼠体内 2 - 3 周,会观察到相同的特异性模式。因此,涉及的是不同的 B 细胞亚群,而非单一群体的不同成熟阶段。在测量对 LPS 和 NWSM 的促有丝分裂原反应之前,用各种同种异体抗血清和补体进行处理表明:(a)所有对 LPS 有反应的细胞都携带表面 Ig,而对 NWSM 有反应的细胞亚群则不携带;(b)对 LPS 和 NWSM 有反应的细胞都携带 I - A 抗原,但可能不携带 I - E 或 I - J 抗原;(c)所有对 LPS 有反应的细胞都携带 I - C 抗原,而大约 25%对 NWSM 有反应的细胞则不携带;(d)存在一个对 NWSM 有反应的细胞亚群,既不携带表面 Ig 也不携带 I - C 抗原,且对抗θ处理有抗性。