Vendrell J P, Rabesandratana H, Huguet M F, Cannat A, Serre A
Infect Immun. 1985 Oct;50(1):310-6. doi: 10.1128/iai.50.1.310-316.1985.
Two lipid-A-free fractions which were extracted from Brucella melitensis and were designated PI and SF stimulated human unsensitized mononuclear cells to proliferate and to secrete immunoglobulins. Both of these effects were observed in cultures of peripheral blood, tonsils, and cord blood lymphocytes. Neither B cells nor T cells alone proliferated in the presence of these fractions, whereas the proliferative response of T cells plus B cells was largely independent of accessory cells. Polyclonal activation was estimated by counting the cells which secreted immunoglobulins of different isotypes into culture supernatants. This phenomenon was strongly T dependent.
从羊布鲁氏菌中提取的两种无脂质A组分,分别命名为PI和SF,可刺激未致敏的人单核细胞增殖并分泌免疫球蛋白。在外周血、扁桃体和脐血淋巴细胞培养物中均观察到了这两种效应。单独的B细胞或T细胞在这些组分存在时均不增殖,而T细胞加B细胞的增殖反应在很大程度上不依赖于辅助细胞。通过计数向培养上清液中分泌不同同种型免疫球蛋白的细胞来估计多克隆激活。这种现象强烈依赖于T细胞。