人血浆中芬氯那酸的葡萄糖醛酸苷酶分析

GLC assay of fenclorac in human plasma.

作者信息

DeLong A F, Polk A, Martin G, Smyth R D, Reavey-Cantwell N H

出版信息

J Pharm Sci. 1978 Aug;67(8):1171-3. doi: 10.1002/jps.2600670841.

Abstract

A simple, sensitive GLC assay for fenclorac is described. Plasma proteins were precipitated with methanol, and the methanolic extract was refluxed with hydrochloric acid to form the methyl esters of fenclorac and the internal standard. The esters were purified by partitioning into benzene. Aliquots of 1 microliter of the concentrated benzene phase were injected into the gas chromatograph and quantitated by a 63Ni-electron-capture detector. Recovery of fenclorac from plasma averaged 82 +/- 1.6%.

摘要

本文描述了一种简单、灵敏的氯氟乙酸气相色谱分析法。用甲醇沉淀血浆蛋白，甲醇提取物用盐酸回流以形成氯氟乙酸和内标的甲酯。通过分配到苯中对酯进行纯化。将1微升浓缩苯相的等分试样注入气相色谱仪，并用63Ni电子捕获检测器进行定量。氯氟乙酸从血浆中的回收率平均为82±1.6%。