Faull K F, Pascoe N, Greene K A, Maddaluno J F, Lunde J, King R J
Nancy Pritzker Laboratory of Behavioral Neurochemistry, Stanford University, School of Medicine, California 94305.
Biomed Environ Mass Spectrom. 1988 Sep;17(3):213-9. doi: 10.1002/bms.1200170310.
Details are presented of an ultra-sensitive gas chromatographic/mass spectrometric assay for phenylacetic acid in plasma and cerebrospinal fluid based on measurements of the relative intensities of the carboxylate anions, derived from the penta- and tetrafluorobenzyl esters under electron capture chemical ionization conditions, of unlabeled and a (13C2)-labeled internal standard. The limits of detection for the penta- and tetrafluorobenzyl esters are 0.85 and 4.0 pg respectively, and the assay is capable of measuring phenylacetic acid concentrations in samples as small as 20 microliter of CSF and plasma. The penta- and tetrafluorobenzyl esters are chromatographically separated on the gas chromatograph column, which allows for their co-injection and independent measurement from the same chromatogram.
本文详细介绍了一种超灵敏气相色谱/质谱分析法，用于测定血浆和脑脊液中的苯乙酸。该方法基于在电子捕获化学电离条件下，对未标记的和一种（13C2）标记的内标物的五氟苄基酯和四氟苄基酯衍生的羧酸根阴离子的相对强度进行测量。五氟苄基酯和四氟苄基酯的检测限分别为0.85和4.0皮克，该分析方法能够测量低至20微升脑脊液和血浆样本中的苯乙酸浓度。五氟苄基酯和四氟苄基酯在气相色谱柱上进行色谱分离，这使得它们可以同时进样，并从同一张色谱图上独立测量。
