Zahran Asmaa M, Mohamed Ismail L, El Asheer Osama M, Tamer Deiaaeldin M, Abo-ELela Mohamed G M, Abdel-Rahim Mona H, El-Badawy Omnia H B, Elsayh Khalid I
1 Department of Clinical Pathology, South Egypt Cancer Institute, Assiut, Egypt.
2 Pediatric Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
Clin Appl Thromb Hemost. 2019 Jan-Dec;25:1076029618825311. doi: 10.1177/1076029618825311.
: Hyperglycemia in type 1 diabetes (T1D) is accompanied by endothelial cell dysfunction which is known to contribute to the pathogenesis of cardiovascular disorders. The aim of the current study was to explore the profile of circulating endothelial progenitor cells (EPCs), circulating endothelial cells (CECs), endothelial and platelet derived micropaticles (EMPs, PMPs) and total microparticles (TMPs), in T1D children in relation to each other and to the metabolic disorders accompanying T1D.
: Thirty T1D patients and 20 age and sex matched healthy volunteers were assessed for HbA1c level and lipid profile. Quantification of CECs, EPCs, TMPs, EMPs and PMPs was done by flow cytometry.
: The mean levels of EMPs, PMPs, TMPs and CECs were significantly higher in diabetic children compared to controls. Meanwhile, the levels of EPCs were significantly lower in diabetic children compared to controls. Both PMPs and CECs showed the highest significant differences between patients and controls and their levels were directly related to HbA1c, total cholesterol, LDL and triglycerides. A moderate correlation was observed between the frequency of PMPs and CECs. EPCs revealed negative correlations with both LDL and triglycerides. TMPs were only related to LDL, while EMPs were only related to HbA1c.
: Although there is disturbance in the levels of EMPs, PMPs, TMPs, CECs and EPCs in type 1 diabetic children compared to the controls, only the levels of PMPs and CECs were closely affected by the poor glycemic control and dyslipidemia occurring in T1D; thus may contribute to a higher risk of cardiovascular diseases.
1型糖尿病（T1D）中的高血糖症伴有内皮细胞功能障碍，已知这会促进心血管疾病的发病机制。本研究的目的是探讨1型糖尿病儿童中循环内皮祖细胞（EPCs）、循环内皮细胞（CECs）、内皮和血小板衍生微粒（EMPs、PMPs）以及总微粒（TMPs）的概况，以及它们之间的相互关系，以及与T1D伴随的代谢紊乱的关系。
对30例T1D患者和20例年龄及性别匹配的健康志愿者进行糖化血红蛋白水平和血脂谱评估。通过流式细胞术对CECs、EPCs、TMPs、EMPs和PMPs进行定量分析。
与对照组相比，糖尿病儿童的EMPs、PMPs、TMPs和CECs平均水平显著更高。同时，与对照组相比，糖尿病儿童的EPCs水平显著更低。PMPs和CECs在患者与对照组之间显示出最高的显著差异，且它们的水平与糖化血红蛋白、总胆固醇、低密度脂蛋白和甘油三酯直接相关。观察到PMPs和CECs频率之间存在中度相关性。EPCs与低密度脂蛋白和甘油三酯均呈负相关。TMPs仅与低密度脂蛋白相关，而EMPs仅与糖化血红蛋白相关。
尽管与对照组相比，1型糖尿病儿童的EMPs、PMPs、TMPs、CECs和EPCs水平存在紊乱，但只有PMPs和CECs的水平受到T1D中血糖控制不佳和血脂异常的密切影响；因此可能导致心血管疾病风险更高。
