Department of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou City, Jiangsu, China.
Institute of Neuroscience, Soochow University, Suzhou City, China.
Epigenomics. 2019 May;11(6):655-667. doi: 10.2217/epi-2018-0187. Epub 2019 Feb 13.
We aim to study the antidepressant mechanism of metformin. Tail suspension test and forced swimming test were used to detect the depression-like behavior; the expressions of target protein were examined by western blot; the levels of target genes were tested by quantitative PCR; the content of α-ketoglutarate and 5hmC were detected by ELISA kit. We showed that metformin can improve the depression-like behavior in spatial restraint stress model; then we found that metformin through AMPK/Tet2 pathway increasing the expression of BDNF to antidepression. Our study provided evidences that metformin plays a role of antidepressant effects through the AMPK/Tet2/BDNF pathway.
我们旨在研究二甲双胍的抗抑郁机制。采用悬尾试验和强迫游泳试验检测抑郁样行为；通过 Western blot 检测靶蛋白的表达；通过 qPCR 检测靶基因的水平；通过 ELISA 试剂盒检测 α-酮戊二酸和 5hmC 的含量。结果表明，二甲双胍可改善空间束缚应激模型中的抑郁样行为；然后我们发现二甲双胍通过 AMPK/Tet2 通路增加 BDNF 的表达来抗抑郁。本研究为二甲双胍通过 AMPK/Tet2/BDNF 通路发挥抗抑郁作用提供了证据。
