紫胶红色素对生物膜的抗菌活性。

Violacein antimicrobial activity on biofilm.

机构信息

Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Ceará, Brazil.

Department of Organic Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.

出版信息

Nat Prod Res. 2020 Dec;34(23):3414-3417. doi: 10.1080/14786419.2019.1569654. Epub 2019 Feb 13.

DOI:10.1080/14786419.2019.1569654

PMID:30760047

Abstract

The aim of this study was to evaluate the antimicrobial potential of violacein (VIO) on biofilm. The minimum biofilm inhibition concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were determined, as well as the effect of VIO exposure time on microbial viability in mature biofilm. Violacein showed good antibiofilm action, inhibiting biofilm formation and eradicating mature biofilm of at concentrations of 20 μg.mL and 160 μg.mL, respectively. At concentrations equal to MBEC and 2x MBEC, the biofilm was eradicated in 3 h and 2h30min of incubation, respectively.When evaluating VIO modulating effect on the action of clinically-used drugs (vancomycin, cefepime, ciprofloxacin and meropenem), especial synergism was observed in the violacein-ciprofloxacin association, it can completely erradicated the mature biofilm at the concentration of 1/2xMBEC and 1/4xMBEC, respectively. VIO shows good antimicrobial action on biofilm and has the potential to synergistically modulate the activity of clinically-used antimicrobials.

摘要

本研究旨在评估紫色素（VIO）对生物膜的抗菌潜力。测定了最低生物膜抑制浓度（MBIC）和最低生物膜清除浓度（MBEC），以及 VIO 暴露时间对成熟生物膜中微生物活力的影响。VIO 在 20μg·mL 和 160μg·mL 浓度下分别表现出良好的抗生物膜作用，抑制生物膜形成并清除成熟生物膜。在 MBEC 和 2x MBEC 浓度下，孵育 3 小时和 2 小时 30 分钟后，生物膜被清除。在评估 VIO 对临床用药（万古霉素、头孢吡肟、环丙沙星和美罗培南）作用的调节作用时，观察到 VIO-环丙沙星联合用药特别具有协同作用，其浓度分别为 1/2xMBEC 和 1/4xMBEC 时可完全清除成熟生物膜。VIO 对生物膜具有良好的抗菌作用，并有潜力协同调节临床使用的抗菌药物的活性。