Arito H, Iino M, Takagi S F
J Physiol. 1978 Jun;279:605-19. doi: 10.1113/jphysiol.1978.sp012364.
The water response elicited by application of distilled water on the olfactory epithelium was recorded extracellularly from single olfactory bulb neurones. Characteristics of the water response in the frog olfactory epithelium were examined in comparison with those of the water response in the gustatory and palatal organs. 1. Effects of various electrolyte solutions on the generation of the water response were studied by dripping distilled water on the olfactory epithelium after adaptation to each of these electrolyte solutions. Number of the olfactory bulb cells responding to distilled water increased with increasing the charge of the adapting cations and also with decreasing the size of the cations with a few exceptions. 2. Magnitude of the 'water response' increased with decreasing concentration of salt in the solution which was dripped after adaptation to the isotonic solution of the same salt. 3. The water response was effectively depressed by an electrolyte solution but not by a non-electrolyte solution. An electrolyte also depressed effectively the water response which was produced after adaptation to an organic salt solution. 4. The water response was blocked by treatment of the olfactory epithelium with the uranyl ions which had high affinity for phospholipids. A tentative hypothesis on the generating mechanism of the water response in the frog olfactory epithelium is presented on the basis of the present experimental results and the water responses of the gustatory and palatal organs so far reported.
通过在嗅上皮上滴加蒸馏水所引发的水反应，是从单个嗅球神经元进行细胞外记录的。将蛙嗅上皮中的水反应特征与味觉和腭部器官中的水反应特征进行了比较研究。1. 在适应各种电解质溶液后，通过向嗅上皮滴加蒸馏水来研究不同电解质溶液对水反应产生的影响。除少数例外情况外，对蒸馏水有反应的嗅球细胞数量随着适应阳离子电荷数的增加以及阳离子尺寸的减小而增加。2. 在适应相同盐的等渗溶液后，滴加的溶液中盐浓度降低，“水反应”的幅度增大。3. 电解质溶液能有效抑制水反应，而非电解质溶液则不能。一种电解质也能有效抑制在适应有机盐溶液后产生的水反应。4. 用对磷脂具有高亲和力的铀离子处理嗅上皮会阻断水反应。基于目前的实验结果以及迄今报道的味觉和腭部器官的水反应，提出了关于蛙嗅上皮中水反应产生机制的初步假说。
