Cutler S J
Microbiology, Stoke Mandeville Hospital, Aylesbury, Bucks.
Tubercle. 1988 Sep;69(3):197-203. doi: 10.1016/0041-3879(88)90022-0.
The enhancement of the growth rate of 11 clinical isolates of mycobacteria, covering six species, were investigated in vials of Middlebrook 7H12 medium which had previously been heated to 60 degrees C, 70 degrees C or 80 degrees C. The optimal temperature which enhanced growth was 70 degrees C. The enhancing effect remained stable for over 3 months. Trypsin treatment of the medium abolished the enhancing effect of heat, but this could be restored by the addition of heated or unheated bovine serum albumin. Protein electrophoresis demonstrated altered electrophoretic mobility of heated bovine serum albumin. It is suggested that this altered bovine serum albumin is the component which promotes enhanced mycobacterial growth.
在先前已加热至60摄氏度、70摄氏度或80摄氏度的Middlebrook 7H12培养基小瓶中，对涵盖六个物种的11株临床分枝杆菌分离株的生长速率增强情况进行了研究。促进生长的最佳温度为70摄氏度。这种促进作用在3个多月内保持稳定。用胰蛋白酶处理培养基可消除热的促进作用，但添加加热或未加热的牛血清白蛋白可恢复这种作用。蛋白质电泳显示加热后的牛血清白蛋白电泳迁移率发生了改变。提示这种改变的牛血清白蛋白是促进分枝杆菌生长增强的成分。
