Middleton A M, Chadwick M V, Gaya H
Department of Microbiology, Royal Brompton Hospital, London, UK.
Eur J Clin Microbiol Infect Dis. 1997 Feb;16(2):166-70. doi: 10.1007/BF01709480.
The rate of recovery and time to detection of mycobacteria from clinical specimens were analysed for specimens inoculated into both radiometric Middlebrook 7H12 (Bactec 12B, originally 12A) medium and Lowenstein-Jensen egg-based medium (with and without sodium pyruvate) over the 15-year period from 1980 to 1994. A total of 19,679 Bactec vials were inoculated together with Lowenstein-Jensen slopes, with 2198 mycobacterial isolates detected. The mean times to detection for Bactec and Lowenstein-Jensen were 11.7 days and 23.9 days, respectively. In 195 cases mycobacteria were isolated from Bactec and not from Lowenstein-Jensen, whereas the reverse was true in 42 cases. The number of contaminated Bactec vials was 488 and the number of contaminated Lowenstein-Jensen slopes 448.
对1980年至1994年这15年间接种于放射性米氏7H12培养基（Bactec 12B，原为12A）和罗氏鸡蛋培养基（含和不含丙酮酸钠）的临床标本中分枝杆菌的恢复率和检测时间进行了分析。共接种了19679个Bactec瓶和罗氏斜面，检测到2198株分枝杆菌分离株。Bactec和罗氏培养基的平均检测时间分别为11.7天和23.9天。195例中，分枝杆菌从Bactec分离而未从罗氏培养基分离，反之，42例则相反。污染的Bactec瓶数量为488个，污染的罗氏斜面数量为448个。
