Department of Cardiology Beijing Children's Hospital Capital Medical University Beijing China.
Department of Pediatrics Beijing Tongren Hospital Capital Medical University Beijing China.
FEBS Open Bio. 2018 Dec 20;9(2):265-275. doi: 10.1002/2211-5463.12563. eCollection 2019 Feb.
Kawasaki disease (KD) is an acute systemic vasculitis that mainly afflicts infants and young children. The symptoms of KD are similar to those of various febrile diseases. Here, we attempted to develop accurate diagnostic biomarkers of KD by performing urine proteomic analysis of samples from healthy controls, patients with KD, and patients with another febrile disease, pneumonia (two patients). We identified differentially expressed proteins (DEPs) in KD as compared to normal controls. We also constructed functional annotation and protein-protein interaction (PPI) networks of DEPs in KD and pneumonia. DEPs common to both KD and pneumonia were identified, as well as DEPs specific to KD. Compared to normal control, 43 and 62 DEPs were identified in KD and pneumonia, respectively. Serine hydroxymethyltransferase 1 is a hub protein of the KD-specific PPI network. Thirteen DEPs common to both KD and pneumonia and 30 DEPs specific to KD were identified. Of these, the expression of eight DEPs could cluster normal and pneumonia samples into one group and cluster KD samples into another group based on hierarchical clustering. Our study identified several DEPs that may play a role in KD and that may serve as diagnostic biomarkers to distinguish patients with KD from both normal control and other febrile diseases.
川崎病（KD）是一种主要影响婴儿和幼儿的急性全身性血管炎。KD 的症状与各种发热性疾病相似。在这里，我们通过对健康对照、KD 患者和另一种发热性疾病肺炎（两名患者）患者的尿液进行蛋白质组学分析，试图开发 KD 的准确诊断生物标志物。我们鉴定了 KD 与正常对照相比的差异表达蛋白（DEPs）。我们还构建了 KD 和肺炎中 DEPs 的功能注释和蛋白质-蛋白质相互作用（PPI）网络。鉴定了 KD 和肺炎共有的 DEPs，以及 KD 特异性的 DEPs。与正常对照相比，KD 和肺炎中分别鉴定出 43 个和 62 个 DEPs。丝氨酸羟甲基转移酶 1 是 KD 特异性 PPI 网络的枢纽蛋白。鉴定出 13 个 KD 和肺炎共有的 DEPs 和 30 个 KD 特异性的 DEPs。其中，基于层次聚类，有 8 个 DEPs 的表达可以将正常和肺炎样本聚类为一组，并将 KD 样本聚类为另一组。我们的研究鉴定了一些可能在 KD 中发挥作用的 DEPs，它们可能作为诊断生物标志物，将 KD 患者与正常对照和其他发热性疾病区分开来。
