Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
Molecular Science and Biomedicine Laboratory, Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
Small. 2019 Aug;15(32):e1804969. doi: 10.1002/smll.201804969. Epub 2019 Feb 14.
Luminescent nanomaterials have attracted great attention in luminescence-based bioanalysis due to their abundant optical and tunable surface physicochemical properties. However, luminescent nanomaterials often suffer from serious autofluorescence and light scattering interference when applied to complex biological samples. Time-resolved luminescence methodology can efficiently eliminate autofluorescence and light scattering interference by collecting the luminescence signal of a long-lived probe after the background signals decays completely. Lanthanides have a unique [Xe]4f electronic configuration and ladder-like energy states, which endow lanthanide-doped nanoparticles with many desirable optical properties, such as long luminescence lifetimes, large Stokes/anti-Stokes shifts, and sharp emission bands. Due to their long luminescence lifetimes, lanthanide-doped nanoparticles are widely used for high-sensitive biosensing and high-contrast bioimaging via time-resolved luminescence methodology. In this review, recent progress in the development of lanthanide-doped nanoparticles and their application in time-resolved biosensing and bioimaging are summarized. At the end of this review, the current challenges and perspectives of lanthanide-doped nanoparticles for time-resolved bioapplications are discussed.
发光纳米材料由于其丰富的光学性质和可调的表面物理化学性质，在基于发光的生物分析中引起了极大的关注。然而，当应用于复杂的生物样品时，发光纳米材料常常受到严重的自发荧光和光散射干扰。时间分辨发光方法通过在背景信号完全衰减后收集长寿命探针的发光信号，可以有效地消除自发荧光和光散射干扰。镧系元素具有独特的 [Xe]4f 电子构型和梯状能级结构，这赋予了镧系掺杂纳米粒子许多理想的光学性质，如长的发光寿命、大的斯托克斯/反斯托克斯位移和尖锐的发射带。由于其长的发光寿命，镧系掺杂纳米粒子被广泛用于通过时间分辨发光方法进行高灵敏度的生物传感和高对比度的生物成像。在本文中，总结了镧系掺杂纳米粒子的最新进展及其在时间分辨生物传感和生物成像中的应用。在本文的最后，讨论了镧系掺杂纳米粒子在时间分辨生物应用中的当前挑战和展望。
