Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.
Chemical Imaging Infrastructure, CII, University of Gothenburg and Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.
Chemistry. 2019 Apr 11;25(21):5406-5411. doi: 10.1002/chem.201900010. Epub 2019 Mar 13.
A micromolar concentration of zinc has been shown to significantly change the dynamics of exocytosis as well as the vesicle contents in a model cell line, providing direct evidence that zinc regulates neurotransmitter release. To provide insight into how zinc modulates these exocytotic processes, neurotransmitter release and vesicle content were compared with single cell amperometry and intracellular impact vesicle cytometry with a range of zinc concentrations. Additionally, time-of-flight secondary ion mass spectrometry (ToF-SIMS) images of lipid distributions in the cell membrane after zinc treatment correlate to changes in exocytosis. By combining electrochemical techniques and mass spectrometry imaging, we proposed a mechanism by which zinc changes the fusion pore and the rate of neurotransmitter release by changing lipid distributions and results in the modulation of synaptic strength and plasticity.
已经证明，微摩尔浓度的锌可以显著改变模型细胞系中胞吐作用的动力学以及囊泡内容物，这为锌调节神经递质释放提供了直接证据。为了深入了解锌如何调节这些胞吐过程，我们在一系列锌浓度下，通过单细胞安培法和细胞内冲击囊泡细胞术比较了神经递质释放和囊泡含量。此外，锌处理后细胞膜中脂质分布的飞行时间二次离子质谱（ToF-SIMS）图像与胞吐作用的变化相关。通过结合电化学技术和质谱成像，我们提出了一种机制，即锌通过改变脂质分布来改变融合孔和神经递质释放的速率，从而导致突触强度和可塑性的调节。
