Xiao Jianhao, Yuan Qian, Zhang Simiao, Li Xiaodong, Bai Hongying, Wang Yunliang, Duan Shiwei
Department of Neurology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan.
Department of Neurology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Pudong, Shanghai.
Medicine (Baltimore). 2019 Feb;98(7):e14593. doi: 10.1097/MD.0000000000014593.
Telomere length is closely related to the onset and prognosis of ischemic stroke. This study was to investigate the relationship between telomere length and the incidence of ischemic stroke in Han population of northern China.
In the present study, 152 patients with ischemic stroke were selected as the case group, and 152 healthy persons were used as the control group. Detection of telomere length was done by real-time polymerase chain reaction after extraction of genomic DNA from peripheral venous blood.
Our results showed that the telomere length of the patients in the case group was significantly lower than that of the control group (Z = -11.843, P < .0001). Further analysis found that the telomere length of the control group was inversely correlated with age (r = -0.234, P = .004), and the telomere length and homocysteine (HCY) were inversely correlated in the case group (r = -0.176, P = .03), especially in women (r = -0.357, P = .024). Multivariate regression analysis showed that telomere length was a protective factor for ischemic stroke (odds ratio [OR] 95% confidence interval [95% CI] = 0.748 [0.681-0.823], β = -0.29, P < .0001). The receiver operating characteristic curve showed that telomere length was a good diagnostic biomarker of ischemic stroke (area under the curve: 0.894, sensitivity: 84.7%, specificity: 93.4%).
Our results indicate that shorter telomere length has some connection with the risk of ischemic stroke in the northern Chinese Han population. Telomere length might serve as a potential candidate biomarker for ischemic stroke. This requires a large sample to be further verified.
端粒长度与缺血性脑卒中的发病及预后密切相关。本研究旨在探讨中国北方汉族人群中端粒长度与缺血性脑卒中发病率之间的关系。
在本研究中，选取152例缺血性脑卒中患者作为病例组，152名健康人作为对照组。从外周静脉血中提取基因组DNA后，采用实时聚合酶链反应检测端粒长度。
我们的结果显示，病例组患者的端粒长度显著低于对照组（Z = -11.843，P <.0001）。进一步分析发现，对照组的端粒长度与年龄呈负相关（r = -0.234，P =.004），病例组中端粒长度与同型半胱氨酸（HCY）呈负相关（r = -0.176，P =.03），尤其是在女性中（r = -0.357，P =.024）。多因素回归分析显示，端粒长度是缺血性脑卒中的保护因素（比值比[OR] 95%置信区间[95%CI] = 0.748 [0.681 - 0.823]，β = -0.29，P <.0001）。受试者工作特征曲线显示，端粒长度是缺血性脑卒中的良好诊断生物标志物（曲线下面积：0.894，敏感性：84.7%，特异性：93.4%）。
我们的结果表明，较短的端粒长度与中国北方汉族人群缺血性脑卒中的风险存在一定关联。端粒长度可能是缺血性脑卒中的潜在候选生物标志物。这需要大样本进一步验证。
