College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.
Department of Geratology, The Third People's Hospital of Hangzhou, Hangzhou, China.
Biofactors. 2019 May;45(3):439-449. doi: 10.1002/biof.1495. Epub 2019 Feb 14.
The aim of the study was to address the causality links and identify specific features of the gut microbiota signatures contributing to host lipids metabolism in the presence or absence of polyene phosphatidylcholine (PPC) administration, and evaluate potential risk of PPC consumption. About 20 C57BL/6J mice were randomly allocated into two groups, normal diet group (CK) and PPC administration group (205.2 mg/kg). Compared with CK group, the contents of unsaturated fatty acids were increased and the saturated fatty acids were decreased in PPC group. The content of free fatty acids (FFA) and lipopolysaccharides (LPS) were significantly decreased (P < 0.05), and expression of carnitine palmitoyltransferase 1A (CPT1A), cluster of differentiation 36 (CD36), liver fatty acid binding protein (L-FABP), fatty acid transport protein 5 (FATP5), and fatty acid synthase (FASN) were significantly decreased in the mRNA and protein levels after treated by PPC (P < 0.05, P < 0.01). Also, we found that acetic acid in feces was significantly increased after consumption of PPC (P < 0.05). After PPC administration the relative abundances of Firmicutes and Clostridia were increased within the phylum level and the class level, respectively. Microbial abundances in genus level were dominated by Lachnospiraceae and Lachnospiraceae_NK4A136_group, whereas the proportion of sequences assigned to Bacteroidetes within the phylum level, class Bacteroidias and Mollicutes, order Anaeroplasmatalesl, genus Bacteroidales_S24-7_group were decreased in metagenomes of treated group with PPC and did not significantly influence on the accumulation of trimethylamine-N-oxide (TMAO). This study revealed that intake of PPC could regulate the gut microbiota signatures and lipids metabolism in mice without TMAO accumulations. © 2019 BioFactors, 45(3):439-449, 2019.
本研究旨在探讨肠道微生物群特征与宿主脂质代谢之间的因果关系，并确定在添加或不添加多烯磷脂酰胆碱（PPC）的情况下，促进宿主脂质代谢的特定特征，同时评估 PPC 消费的潜在风险。大约 20 只 C57BL/6J 小鼠被随机分为两组，正常饮食组（CK）和 PPC 给药组（205.2mg/kg）。与 CK 组相比，PPC 组不饱和脂肪酸含量增加，饱和脂肪酸含量减少。游离脂肪酸（FFA）和脂多糖（LPS）含量显著降低（P<0.05），肉碱棕榈酰转移酶 1A（CPT1A）、分化群 36（CD36）、肝脂肪酸结合蛋白（L-FABP）、脂肪酸转运蛋白 5（FATP5）和脂肪酸合成酶（FASN）的表达在 mRNA 和蛋白水平上均显著降低（P<0.05，P<0.01）。此外，我们发现 PPC 消耗后粪便中乙酸含量显著增加（P<0.05）。PPC 给药后，厚壁菌门和梭菌纲的相对丰度分别在门水平和纲水平上增加。属水平上的微生物丰度主要由 Lachnospiraceae 和 Lachnospiraceae_NK4A136_group 主导，而拟杆菌门、拟杆菌纲和 Mollicutes 纲的比例在门水平上下降，Anaeroplasmatalesl、Bacteroidales_S24-7_group 的序列比例在属水平上下降在 PPC 处理组的宏基因组中，但对三甲胺 N-氧化物（TMAO）的积累没有显著影响。本研究表明，PPC 的摄入可以调节小鼠的肠道微生物群特征和脂质代谢，而不会导致 TMAO 的积累。
