Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Jinan, China.
The Laboratory of Food Nutrition and Functional Factors, School of Food Science and Technology, Jiangnan University, Wuxi, China.
Eur J Nutr. 2019 Oct;58(7):2625-2638. doi: 10.1007/s00394-018-1810-2. Epub 2018 Aug 17.
Previous studies have shown that industrially originated trans-fatty acids (iTFAs) are associated with several chronic diseases, but the underlying mechanisms remain unknown. Because gut microbiota play a critical role in human health, diet competent induced gut microbiota dysbiosis may contributing to disease pathogenesis. Therefore, the present study examined the impact of iTFA on gut microbiota, help understanding the underling mechanism of iTFA-associated chronic diseases.
Forty male 8-week-old mice were divided into 4 groups and randomly assigned to diets containing soybean oil (non-iTFA) or partially hydrogenated soybean oil (iTFA). The intervention groups were: (1) low soybean oil (LS); (2) high soybean oil (HS); (3) low partially hydrogenated oil (LH) and (4) high partially hydrogenated oil (HH). The gut microbiota profiles were determined by 16S rRNA gene sequencing. Physiological parameters and the inflammatory status of the small intestine and other tissues were analyzed. Short-chain fatty acid levels in feces were measured using gas chromatography.
The intake of iTFA increased the abundance of well-documented 'harmful' bacteria, such as Proteobacteria and Desulfovibrionaceae (P < 0.05), whereas it decreased relative abundance of 'beneficial' bacteria, such as Bacteroidetes, Lachnospiraceae, Bacteroidales S24-7 (P < 0.05). Surprisingly, the intake of iTFA increased the abundance of the probiotic Lactobacillaceae (P < 0.05). Additionally, the intake of iTFA induced increase of inflammatory parameters, as well as a numerical decrease of fecal butyric acid and valeric acid.
This study, to our knowledge, is the first to demonstrate that the consumption of iTFA resulted in a significant dysbiosis of gut microbiota, which may contribute to the development of chronic diseases associated with iTFA.
先前的研究表明，工业来源的反式脂肪酸（iTFA）与多种慢性疾病有关，但潜在机制尚不清楚。由于肠道微生物群在人类健康中起着至关重要的作用，饮食相关的肠道微生物群失调可能导致疾病的发病机制。因此，本研究旨在研究 iTFA 对肠道微生物群的影响，以帮助了解 iTFA 相关慢性疾病的潜在机制。
将 40 只 8 周龄雄性小鼠分为 4 组，并随机分配到含有豆油（非 iTFA）或部分氢化豆油（iTFA）的饮食中。干预组分别为：（1）低豆油（LS）；（2）高豆油（HS）；（3）低部分氢化油（LH）和（4）高部分氢化油（HH）。通过 16S rRNA 基因测序确定肠道微生物群谱。分析生理参数和小肠及其他组织的炎症状态。使用气相色谱法测量粪便中短链脂肪酸水平。
iTFA 的摄入增加了已被充分证实的“有害”细菌的丰度，如变形菌门和脱硫弧菌科（P < 0.05），而有益细菌的相对丰度，如拟杆菌门、lachnospiraceae、拟杆菌目 S24-7（P < 0.05）下降。令人惊讶的是，iTFA 的摄入增加了益生菌乳杆菌科的丰度（P < 0.05）。此外，iTFA 的摄入诱导了炎症参数的增加，以及粪便丁酸和戊酸数量的减少。
本研究首次证明，iTFA 的摄入导致肠道微生物群的显著失调，这可能有助于 iTFA 相关慢性疾病的发展。
