Department of Animal Science, Aarhus University, Blichers Allé 20, 8830, Tjele, Denmark.
GABI, INRA, AgroParisTech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Dev Comp Immunol. 2019 Jul;96:93-102. doi: 10.1016/j.dci.2019.02.004. Epub 2019 Feb 11.
Vaccination programs are implemented in poultry farms to limit outbreaks and spread of infectious bronchitis virus (IBV), which is a substantial economic burden in the poultry industry. Immune correlates, used to predict vaccine efficacy, have proved difficult to find for IBV-vaccine-induced protection. To find correlates of IBV-vaccine-induced protection, hence, we employed a flow cytometric assay to quantify peripheral leucocyte subsets and expression of cell surface markers of six different non-vaccinated and vaccinated Major Histocompatibility Complex (MHC) haplotypes. Non-vaccinated and vaccinated MHC haplotypes presented differential leucocyte composition and IBV viral load. A strong effect of MHC-B, but not vaccination, on several leucocyte subsets resulted in positive correlations with IBV viral load based on MHC haplotype ranking. In addition, a strong effect of MHC-B and vaccination on monocyte MHC-II expression showed that animals with highest monocyte MHC-II expression had weakest vaccine-induced protection. In conclusion, we found several interesting MHC-B related immune correlates of protection and that flow cytometric analysis can be employed to study correlates of IBV-vaccine-induced protection.
在禽类养殖场实施疫苗接种计划，以限制传染性支气管炎病毒（IBV）的爆发和传播，该病毒给禽类产业带来了巨大的经济负担。免疫相关性指标曾被用于预测疫苗的效果，但已证明难以找到用于 IBV 疫苗诱导保护的相关性指标。因此，为了寻找 IBV 疫苗诱导保护的相关性指标，我们采用流式细胞术检测方法，定量分析了六个不同未接种疫苗和接种疫苗的主要组织相容性复合体（MHC）单倍型的外周白细胞亚群和细胞表面标志物的表达。未接种疫苗和接种疫苗的 MHC 单倍型呈现出不同的白细胞组成和 IBV 病毒载量。MHC-B 而非疫苗接种对几种白细胞亚群的强烈影响，根据 MHC 单倍型的排名，与 IBV 病毒载量呈正相关。此外，MHC-B 和疫苗接种对单核细胞 MHC-II 表达的强烈影响表明，单核细胞 MHC-II 表达最高的动物具有最弱的疫苗诱导保护作用。总之，我们发现了一些与 MHC-B 相关的有趣的保护免疫相关性指标，并且流式细胞术分析可以用于研究 IBV 疫苗诱导保护的相关性指标。
