a Department of Biochemistry and Molecular Biology Faculty of Medicine , University of Debrecen , Debrecen , Hungary.
b Institute of Sport Sciences University of Debrecen , Debrecen , Hungary.
Int J Food Sci Nutr. 2019 Sep;70(6):749-758. doi: 10.1080/09637486.2018.1557607. Epub 2019 Feb 15.
Dietary food, depending on timing, amount and composition can influence gene expression in various tissues. Here, we investigated the effect of high-fat meal diets of different compositions on the gene expression pattern of human skeletal muscle. Gene expression data of skeletal muscle samples from human volunteers prior and 4 h after the consumption of high lipid-containing meal consisting of either saturated-, monounsaturated- or polyunsaturated fatty acids were downloaded from the public repository. List of 843 differently expressed genes (DEGs) was generated. Functional analysis revealed that circadian rhythm-, inflammation- and oxidative stress-related genes are highly overrepresented among the DEGs. The magnitude of gene expression changes significantly increases with the saturation level of the dietary fatty acids and the majority of the DEGs are upregulated. We propose that, by altering circadian clock gene expression and inducing inflammation and oxidative stress, high lipid intake can contribute to muscle function decay in the long run.
饮食食物，取决于时间、数量和组成，可能会影响各种组织中的基因表达。在这里，我们研究了不同组成的高脂肪膳食对人体骨骼肌基因表达模式的影响。从公共数据库中下载了人类志愿者在摄入富含脂质的膳食（含饱和脂肪、单不饱和脂肪或多不饱和脂肪）前后 4 小时的骨骼肌样本的基因表达数据。生成了 843 个差异表达基因（DEGs）列表。功能分析表明，昼夜节律、炎症和氧化应激相关基因在 DEGs 中高度过表达。基因表达变化的幅度随着膳食脂肪酸的饱和度显著增加，并且大多数 DEGs 呈上调。我们提出，通过改变生物钟基因表达并诱导炎症和氧化应激，高脂肪摄入可能会导致肌肉功能随着时间的推移而衰退。
