Wu Yarong, Lin Tianran, Huang Jianli, Chen Yi, Zhong Jianhai, Guo Liangqia, Fu Fengfu
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and College of Chemistry, Fuzhou University, Fuzhou, Fujian 3501168, China.
Institute of Grain and Oil Quality Supervision and Test of Fujian, Fuzhou 350012, China.
J Nanosci Nanotechnol. 2019 Jul 1;19(7):4220-4227. doi: 10.1166/jnn.2019.16293.
Boron- and phenyl-doped graphitic carbon nitride nanosheets (BPCN NSs) were prepared by thermal polymerization of cyanamide with 3-aminobenzeneboronic acid followed by ultrasonic exfoliation. BPCN NSs exhibited enhanced peroxidase-like activity and catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine by H₂O₂. A simple sensitive colorimetric senor was developed for H₂O₂ by utilizing TMB as the substrate and BPCN NSs as enzyme mimetic. The linear relations between the absorbance and H₂O₂ concentration over the range from 0 to 280 mol L and from 280 to 1000 mol L were obtained with the limit of detection of 1.0 mol L according to the 3 rule. The colorimetric sensor was applied for the detection of H₂O₂ residue in simulated soaked foods with satisfied results. Finally, the portable test kits for H₂O₂ were prepared and applied for the semi-quantitative assay of H₂O₂ residues in soaked chicken feet.
通过氰胺与3-氨基苯硼酸热聚合，随后超声剥离制备了硼和苯基掺杂的石墨相氮化碳纳米片（BPCN NSs）。BPCN NSs表现出增强的过氧化物酶样活性，并催化过氧化氢对3,3',5,5'-四甲基联苯胺（TMB）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）和邻苯二胺的氧化。以TMB为底物，BPCN NSs为酶模拟物，开发了一种用于过氧化氢的简单灵敏比色传感器。根据3σ规则，在0至280 μmol L和280至1000 μmol L范围内获得了吸光度与过氧化氢浓度之间的线性关系，检测限为1.0 μmol L。该比色传感器用于检测模拟浸泡食品中的过氧化氢残留，结果令人满意。最后，制备了过氧化氢便携式检测试剂盒，并用于浸泡鸡爪中过氧化氢残留的半定量测定。
