Chen Lina, Song Jia, Wang Jun, Ye Mao, Deng Qianqian, Wu Xiaobao, Wu Xiaoyi, Ren Bing
The Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550005, China.
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Guizhou University, Guiyang 550025, China.
Insects. 2023 Jan 31;14(2):145. doi: 10.3390/insects14020145.
Methyl jasmonate (MeJA) is a volatile substance derived from jasmonic acid (JA), and it responds to interbiotic and abiotic stresses by participating in interplant communication. Despite its function in interplant communication, the specific role of MeJA in insect defense responses is poorly understood. In this study, we found that carboxylesterase (CarE) activities, glutathione-S-transferase (GSTs) activities, and cytochrome mono-oxygenases (P450s) content increased more after the feeding of diets containing xanthotoxin, while larvae exposed to MeJA fumigation also showed higher enzyme activity in a dose-dependent manner: lower and medium concentrations of MeJA induced higher detoxification enzyme activities than higher concentrations of MeJA. Moreover, MeJA improved the growth of larvae fed on the control diet without toxins and diets with lower concentrations of xanthotoxin (0.05%); however, MeJA could not protect the larvae against higher concentrations of xanthotoxin (0.1%, 0.2%). In summary, we demonstrated that MeJA is effective at inducing defense response, but the enhanced detoxifying ability could not overcome the strong toxins.
茉莉酸甲酯（MeJA）是一种源自茉莉酸（JA）的挥发性物质，它通过参与植株间通讯对生物间和非生物胁迫做出响应。尽管其在植株间通讯中发挥作用，但MeJA在昆虫防御反应中的具体作用仍知之甚少。在本研究中，我们发现，在喂食含有花椒毒素的日粮后，羧酸酯酶（CarE）活性、谷胱甘肽-S-转移酶（GSTs）活性和细胞色素单加氧酶（P450s）含量增加得更多，而暴露于MeJA熏蒸下的幼虫也呈剂量依赖性地表现出更高的酶活性：较低和中等浓度的MeJA比较高浓度的MeJA诱导更高的解毒酶活性。此外，MeJA促进了以不含毒素的对照日粮和含有较低浓度花椒毒素（0.05%）的日粮为食的幼虫的生长；然而，MeJA无法保护幼虫免受较高浓度花椒毒素（0.1%、0.2%）的侵害。总之，我们证明了MeJA在诱导防御反应方面是有效的，但增强的解毒能力无法克服强毒素。
Zotero 插件
