Department of Biotransformation, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Faculty of Chemistry, University of Wroclaw, Joliot-Curie 14, 50-383 Wroclaw, Poland.
Bioorg Chem. 2019 Dec;93:102787. doi: 10.1016/j.bioorg.2019.02.012. Epub 2019 Feb 11.
Levan is a polysaccharide composed of fructose units with β-2,6-glycoside bonds. Microorganisms synthesize levan by levansucrase as a mixture of low- and high-molecular-weight fractions. Due to its properties, it has a wide range of applications in cosmetics, pharmaceuticals, food and medicine; it appears that the molecular weight of levan might impact its industrial use. To obtain one fraction of levan after biotransformation, ethanol precipitation with an increasing volume of alcohol was conducted. This precipitation process was also optimized. Several types of analyses were used. Low-molecular-weight levan was evaluated for toxicity in a normal human dermal fibroblast cell line and hemolytic potential on human erythrocytes. Levan was found to be non-cytotoxic and non-hemolytic in concentrations ranging from 0.01 to 1.00 mg/ml. Moreover, levan demonstrated antioxidant potential expressed as an ability to inhibit of oil/water emulsion oxidation and DPPH radical scavenging.
蔗聚糖是由果糖单元组成的多糖，具有β-2,6-糖苷键。微生物通过蔗糖酶合成分散的低分子量和高分子量蔗聚糖混合物。由于其特性，它在化妆品、制药、食品和医药领域有广泛的应用；似乎蔗聚糖的分子量可能会影响其工业用途。为了在生物转化后获得蔗聚糖的单一馏分，用逐渐增加的酒精体积进行了乙醇沉淀。还优化了这个沉淀过程。使用了几种类型的分析。在正常人真皮成纤维细胞系中评估了低分子量蔗聚糖的毒性和对人红细胞的溶血潜力。在 0.01 至 1.00 mg/ml 的浓度范围内，发现蔗聚糖既无细胞毒性也不溶血。此外，蔗聚糖表现出抗氧化潜力，能够抑制油/水乳液氧化和 DPPH 自由基清除。
