González-Torres Mariana, Hernández-Rosas Francisco, Pacheco Neith, Salinas-Ruiz Josafhat, Herrera-Corredor José A, Hernández-Martínez Ricardo
Colegio de Postgraduados, Campus Córdoba, Carretera Federal Córdoba-Veracruz Federal Km 348, Congregación Manuel León, Municipio Amatlán de los Reyes, Veracruz 94946, Mexico.
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Subsede Sureste, Mérida 97302, Mexico.
Molecules. 2024 Feb 29;29(5):1105. doi: 10.3390/molecules29051105.
The valorization of byproducts from the sugarcane industry represents a potential alternative method with a low energy cost for the production of metabolites that are of commercial and industrial interest. The production of exopolysaccharides (EPSs) was carried out using the yeast isolated from agro-industrial sugarcane, and the products and byproducts of this agro-industrial sugarcane were used as carbon sources for their recovery. The effect of pH, temperature, and carbon and nitrogen sources and their concentration in EPS production by submerged fermentation (SmF) was studied in 170 mL glass containers of uniform geometry at 30 °C with an initial pH of 6.5. The resulting EPSs were characterized with Fourier-transform infrared spectroscopy (FT-IR). The results showed that the highest EPS production yields were 4.26 and 44.33 g/L after 6 h of fermentation using sucrose and molasses as carbon sources, respectively. Finally, an FT-IR analysis of the EPSs produced by corresponded to levan, corroborating its origin. It is important to mention that this is the first work that reports the production of levan using this yeast. This is relevant because, currently, most studies are focused on the use of recombinant and genetically modified microorganisms; in this scenario, is a native yeast isolated from the sugar production process, giving it a great advantage in the incorporation of carbon sources into their metabolic processes in order to produce levan sucrose, which uses fructose to polymerize levan.
甘蔗产业副产品的增值代表了一种潜在的替代方法，该方法能耗低，可用于生产具有商业和工业价值的代谢产物。利用从农业工业甘蔗中分离出的酵母进行胞外多糖（EPS）的生产，并将这种农业工业甘蔗的产品和副产品用作其回收的碳源。在170 mL几何形状均匀的玻璃容器中，于30°C、初始pH值为6.5的条件下，研究了pH、温度、碳源和氮源及其浓度对通过深层发酵（SmF）生产EPS的影响。用傅里叶变换红外光谱（FT-IR）对所得的EPS进行了表征。结果表明，分别以蔗糖和糖蜜为碳源发酵6小时后，EPS的最高产量分别为4.26 g/L和44.33 g/L。最后，对所产生的EPS进行的FT-IR分析表明其对应于果聚糖，证实了其来源。值得一提 的是，这是第一项报道使用这种酵母生产果聚糖的研究。这很重要，因为目前大多数研究都集中在使用重组和转基因微生物；在这种情况下， 是从制糖过程中分离出的天然酵母，这使其在将碳源纳入其代谢过程以生产果聚糖蔗糖方面具有很大优势，果聚糖蔗糖利用果糖聚合成果聚糖。
