Faculty of Optometry, Ramkhamhaeng University, Bangkok, Thailand.
Faculty of Optometry, Ramkhamhaeng University, Bangkok, Thailand
Biosci Rep. 2019 Feb 26;39(2). doi: 10.1042/BSR20182470. Print 2019 Feb 28.
The hepatitis C virus (HCV) is able to cause a life-threatening disease relating to lethal hepatocellular carcinoma. Previous, Toll-like receptor polymorphisms were proposed as promising biomarker for HCV-related hepatocellular carcinoma and disease progression. This study aimed to summarize the association of TLR4 polymorphisms and HCV infection through meta-analysis. We applied a systematic review and meta-analysis performed by using PubMed, EMBASE and Web of Science searches. The Modified Newcastle-Ottawa scale was used for quality assessment. The odd-ratio (OR) and 95% confidence interval (CI) were calculated to assess the association. analysis was applied for proposing the function as microRNA (miRNA) of non-coding polymorphism. Finally, the miRNA target was predicted and annotated to suggest the possible relationship between polymorphism and HCV infection. Our meta-analysis incorporated seven studies involving rs4986791, rs4986790 and rs2149356. No association exists between rs4986791 and HCV infection. However, the heterozygous model (AG vs GG) of rs4986790 significantly associates with HCV infection (OR = 0.33, 95% CI = 0.21-0.49, <0.0001). Moreover, the rs2149356 TG genotype also associates with HCV infection in the over-dominant model (TG vs TT+TG: OR = 0.54, 95% CI = 0.40-0.75). analysis of rs2149356G allele showed that this mutation is siRNA, which targets the set of genes, especially in the autophagy pathway. We demonstrated that rs4986790 and rs2149356 are associated with HCV infection.
丙型肝炎病毒 (HCV) 能够导致危及生命的疾病，与致命的肝细胞癌有关。以前，Toll 样受体多态性被认为是 HCV 相关肝细胞癌和疾病进展的有前途的生物标志物。本研究旨在通过荟萃分析总结 TLR4 多态性与 HCV 感染的相关性。我们应用 PubMed、EMBASE 和 Web of Science 搜索进行系统评价和荟萃分析。使用 Modified Newcastle-Ottawa 量表进行质量评估。计算比值比（OR）和 95%置信区间（CI）来评估相关性。进行 分析以提出非编码多态性作为微小 RNA (miRNA) 的功能。最后，预测 miRNA 靶标并注释以提示多态性与 HCV 感染之间的可能关系。我们的荟萃分析纳入了涉及 rs4986791、rs4986790 和 rs2149356 的 7 项研究。rs4986791 与 HCV 感染之间没有关联。然而，rs4986790 的杂合子模型（AG 与 GG）与 HCV 感染显著相关（OR = 0.33，95%CI = 0.21-0.49，<0.0001）。此外，rs2149356 的 TG 基因型在超显性模型中也与 HCV 感染相关（TG 与 TT+TG：OR = 0.54，95%CI = 0.40-0.75）。rs2149356G 等位基因的 分析表明，这种突变是 siRNA，其靶向一组基因，特别是自噬途径中的基因。我们证明 rs4986790 和 rs2149356 与 HCV 感染相关。
