Department of Entomology, Fund for Citrus Protection (FUNDECITRUS), 14807-040, Araraquara, São Paulo, Brazil.
Centre of Nature Sciences, Federal University of São Carlos (UFSCAR), Buri, São Paulo, Brazil.
Sci Rep. 2019 Feb 14;9(1):2070. doi: 10.1038/s41598-019-38597-5.
Novel, suitable and sustainable alternative control tactics that have the potential to reduce migration of Diaphorina citri into commercial citrus orchards are essential to improve management of huanglongbing (HLB). In this study, the effect of orange jasmine (Murraya paniculata) as a border trap crop on psyllid settlement and dispersal was assessed in citrus orchards. Furthermore, volatile emission profiles and relative attractiveness of both orange jasmine and sweet orange (Citrus × aurantium L., syn. Citrus sinensis (L.) Osbeck) nursery flushes to D. citri were investigated. In newly established citrus orchards, the trap crop reduced the capture of psyllids in yellow sticky traps and the number of psyllids that settled on citrus trees compared to fallow mowed grass fields by 40% and 83%, respectively. Psyllids were attracted and killed by thiamethoxam-treated orange jasmine suggesting that the trap crop could act as a 'sink' for D. citri. Additionally, the presence of the trap crop reduced HLB incidence by 43%. Olfactometer experiments showed that orange jasmine plays an attractive role on psyllid behavior and that this attractiveness may be associated with differences in the volatile profiles emitted by orange jasmine in comparison with sweet orange. Results indicated that insecticide-treated M. paniculata may act as a trap crop to attract and kill D. citri before they settled on the edges of citrus orchards, which significantly contributes to the reduction of HLB primary spread.
新型、适用且可持续的替代控制策略对于减少柑橘黄龙病菌（HLB）的传播至关重要，这些策略具有降低柑橘木虱向商业柑橘果园迁移的潜力。在本研究中，评估了作为边界诱捕作物的橙茉莉（Murraya paniculata）对木虱定殖和扩散的影响。此外，还研究了橙茉莉和甜橙（Citrus × aurantium L.，syn. Citrus sinensis （L.） Osbeck）苗圃新芽对柑橘木虱的挥发物排放谱和相对吸引力。在新建立的柑橘果园中，与休耕割草的草地相比，诱捕作物使黄色粘性陷阱中捕获的木虱数量减少了 40%，在柑橘树上定殖的木虱数量减少了 83%。噻虫嗪处理的橙茉莉对木虱具有吸引力并能杀死它们，这表明诱捕作物可以作为柑橘木虱的“汇”。此外，诱捕作物的存在使 HLB 发病率降低了 43%。嗅觉计实验表明，橙茉莉对木虱的行为具有吸引力，这种吸引力可能与橙茉莉与甜橙相比挥发物谱的差异有关。结果表明，杀虫剂处理的 M. paniculata 可以作为诱捕作物，在柑橘果园边缘吸引并杀死柑橘木虱，从而显著减少 HLB 的初始传播。
