Mashiba H, Matsunaga K, Tomoda H, Furusawa M, Jimi S, Tokunaga T
Department of Gastro-intestinal Surgery, National Kyushu Cancer Center, Fukuoka.
Jpn J Med Sci Biol. 1988 Oct-Dec;41(5-6):197-202. doi: 10.7883/yoken1952.41.197.
Effects of a DNA-rich fraction from Mycobacterium bovis BCG (MY-1) on the natural killer (NK) activity of peripheral blood lymphocytes (PBL) from healthy donors and cancer patients were studied in vitro. The NK activity of PBL was assessed after incubating PBL for 24 hr in the presence or absence of MY-1 or that digested preliminarily with RNase or DNase. One microgram per ml of MY-1 or that digested with RNase augmented the NK activity of PBL from healthy donors. The activity of MY-1 was abolished by the digestion with DNase. Similarly, the NK activity in all of six patients with gastric cancer, 12 patients with colonic cancer, and six patients with uterine cancer was augmented by incubation with MY-1 (1 microgram/ml and 10 micrograms/ml), although the degree of augmentation varied depending upon the origin of PBL.
体外研究了来自卡介苗（MY-1）的富含DNA组分对健康供体和癌症患者外周血淋巴细胞（PBL）自然杀伤（NK）活性的影响。在有或无MY-1或预先用核糖核酸酶或脱氧核糖核酸酶消化的MY-1存在下，将PBL孵育24小时后评估PBL的NK活性。每毫升1微克的MY-1或用核糖核酸酶消化的MY-1增强了健康供体PBL的NK活性。用脱氧核糖核酸酶消化可消除MY-1的活性。同样，6例胃癌患者、12例结肠癌患者和6例子宫癌患者的NK活性在用MY-1（1微克/毫升和10微克/毫升）孵育后均增强，尽管增强程度因PBL来源而异。
