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带有特定回文序列的合成寡核苷酸在体外可刺激人外周血淋巴细胞产生干扰素。

Synthetic oligonucleotides with certain palindromes stimulate interferon production of human peripheral blood lymphocytes in vitro.

作者信息

Yamamoto T, Yamamoto S, Kataoka T, Komuro K, Kohase M, Tokunaga T

机构信息

Department of Bacterial and Blood Products, National Institute of Health, Tokyo.

出版信息

Jpn J Cancer Res. 1994 Aug;85(8):775-9. doi: 10.1111/j.1349-7006.1994.tb02947.x.

DOI:10.1111/j.1349-7006.1994.tb02947.x
PMID:7523351
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5919569/
Abstract

We studied the ability of synthetic single-stranded 30-mer oligodeoxyribonucleotides (oligoDNAs) with three different kinds of hexamer palindromic sequence to induce interferon (IFN) production of human peripheral blood lymphocytes (PBL). When PBL was cultured with oligoDNA having a palindrome of AACGTT or GACGTC, IFN activity was detected by bioassay in the culture fluid after 8 h, and the amount of IFN reached the maximum after 18 h. IFN-alpha was predominantly produced, and small amounts of IFN-beta and IFN-gamma were also found. OligoDNA with the palindrome ACCGGT had no effect.

摘要

我们研究了具有三种不同六聚体回文序列的合成单链30聚体寡脱氧核糖核苷酸（oligoDNA）诱导人外周血淋巴细胞（PBL）产生干扰素（IFN）的能力。当PBL与具有AACGTT或GACGTC回文序列的oligoDNA一起培养时，8小时后通过生物测定法在培养液中检测到IFN活性，18小时后IFN量达到最大值。主要产生IFN-α，也发现少量的IFN-β和IFN-γ。具有ACCGGT回文序列的oligoDNA没有效果。

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Jpn J Cancer Res. 1994 Aug;85(8):775-9. doi: 10.1111/j.1349-7006.1994.tb02947.x.
2
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本文引用的文献

1
Ability of oligonucleotides with certain palindromes to induce interferon production and augment natural killer cell activity is associated with their base length.具有特定回文结构的寡核苷酸诱导干扰素产生及增强自然杀伤细胞活性的能力与其碱基长度有关。
Antisense Res Dev. 1994 Summer;4(2):119-22. doi: 10.1089/ard.1994.4.119.
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