Department of Clinical Laboratory, Bethune International Peace Hospital of PLA, 398 Zhongshan Road, Shijiazhuang, 050082, Hebei, People's Republic of China.
Eur J Clin Microbiol Infect Dis. 2019 May;38(5):891-894. doi: 10.1007/s10096-019-03491-5. Epub 2019 Feb 14.
Candiduria is common in clinical practice. However, an effective and convenient assay to screen for candiduria is still needed. This study aimed to evaluate the performance of the Sysmex UF-1000i urine analyzer for yeast-like cell counting (YLCC) to screen for candiduria prior to urine culture. We retrospectively analyzed data from 5233 urine samples from 1813 patients, including 837 males and 976 females. Urine culture and urinalysis-obtained YLCC data were used to estimate the performance of YLCC in diagnosing candiduria. Different cutoff values were used to calculate sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). The YLCC-positive rates differed according to the Candida colony-forming units (CFU) counts in the urine samples. A sharp drop in YLCC-positive rate (from 64.3 to 22.0%) was observed between the urine groups with 10 CFUs and 10 CFUs. A cutoff value of 0 YLCs/μL results in the highest Youden index (0.71) with 77.04% sensitivity and 93.68% specificity. In a group of 34 hospitalized candiduria patients with serial urinalysis data, 25 were YLCC-positive before urine culture. In conclusion, YLCC with the Sysmax UF-1000i could serve as an auxiliary technique to exclude culture-negative specimens prior to urine culture. Positive YLCC results could imply candiduria, especially when persistent YLCC-positive results were observed.
尿酵母菌计数（YLCC）用于筛查尿培养前的假丝酵母菌尿症：Sysmex UF-1000i 尿沉渣分析仪的性能评估
在临床实践中，尿酵母菌（Candida）属的假丝酵母菌（Candida）属是常见的。然而，仍然需要一种有效且方便的检测方法来筛查假丝酵母菌尿症。本研究旨在评估 Sysmex UF-1000i 尿沉渣分析仪用于酵母样细胞计数（YLCC）筛查尿培养前假丝酵母菌尿症的性能。我们回顾性分析了 1813 例患者的 5233 份尿液样本的数据，其中男性 837 例，女性 976 例。尿培养和尿液分析获得的 YLCC 数据用于估计 YLCC 在诊断假丝酵母菌尿症中的性能。使用不同的截止值计算灵敏度、特异性、阳性预测值（PPV）和阴性预测值（NPV）。根据尿液样本中 Candida 集落形成单位（CFU）的计数，YLCC 阳性率有所不同。在 CFU 为 10 CFUs 和 10 CFUs 的尿液组之间，YLCC 阳性率急剧下降（从 64.3%降至 22.0%）。0 YLCs/μL 的截止值产生最高的 Youden 指数（0.71），具有 77.04%的灵敏度和 93.68%的特异性。在一组 34 例有连续尿液分析数据的住院假丝酵母菌尿症患者中，25 例在尿培养前 YLCC 阳性。总之，Sysmax UF-1000i 的 YLCC 可作为一种辅助技术，在尿培养前排除培养阴性的标本。阳性 YLCC 结果可能提示假丝酵母菌尿症，特别是当持续出现阳性 YLCC 结果时。
