Tian Xiumei, Wang Xiaoli, Peng Shitao, Wang Zhi, Zhou Ran, Tian He
School of Environmental Science & Safety Engineering, Tianjin University of Technology, Tianjin 300384, China E-mail:
Laboratory of Environmental protection in Water Transport Engineering, Tianjin Research Institute for Water Transport Engineering, Ministry of Transport, Tianjin 300456, China.
Water Sci Technol. 2018 Dec;78(12):2626-2638. doi: 10.2166/wst.2019.025.
The aim of this study was to isolate hydrocarbons-degrading bacteria for treatment of oily wastewater from long-standing petroleum-polluted sediments in Bohai Bay, China. Six hydrocarbons-degrading bacteria were screened and identified as Pseudomonas sp. and Bacillus sp. A new approach using a combination of various bacterial species in petroleum biodegradation was proposed and evaluated for its degradation characteristics. Gas chromatography-flame ionization detection (GC-FID) analysis showed that mixed bacterial agents (YJ01) degraded 80.64% of crude oil and 76.30% of crude oil alkanes, exhibiting good biodegradation effect. Besides, after 14 days of culture, the biodegradation assessment markers, pristane and phytane, showed significant degradation rates of 46.75% and 78.23%, respectively. Kinetic analysis indicated that the degradation trends followed a single first-order kinetics model and the degradation half-life (t) of 15 g/L crude oil was significantly shorter (5.48 days). These results indicated that YJ01 could degrade a wider range of hydrocarbons as well as some recalcitrant hydrocarbon components, and can be applied for bioremediation and treatment of oil-contaminated environment.
本研究的目的是从中国渤海湾长期受石油污染的沉积物中分离出可降解碳氢化合物的细菌，用于处理含油废水。筛选出6株可降解碳氢化合物的细菌，鉴定为假单胞菌属和芽孢杆菌属。提出了一种在石油生物降解中使用多种细菌组合的新方法，并对其降解特性进行了评估。气相色谱-火焰离子化检测（GC-FID）分析表明，混合菌剂（YJ01）对原油的降解率为80.64%，对原油烷烃的降解率为76.30%，显示出良好的生物降解效果。此外，培养14天后，生物降解评估标志物姥鲛烷和植烷的降解率分别达到46.75%和78.23%，降解效果显著。动力学分析表明，降解趋势符合单一的一级动力学模型，15 g/L原油的降解半衰期（t）明显较短（5.48天）。这些结果表明，YJ01能够降解更广泛的碳氢化合物以及一些难降解的碳氢化合物成分，可用于石油污染环境的生物修复和处理。
