Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, Maryland, and Detroit, Michigan.
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan.
Am J Reprod Immunol. 2019 May;81(5):e13102. doi: 10.1111/aji.13102. Epub 2019 Mar 29.
The immunophenotype of B cells at the maternal-fetal interface (decidua) in labor at term and preterm labor is poorly understood.
Decidual tissues were obtained from women with preterm or term labor and from non-labor gestational age-matched controls. Immunophenotyping of decidual B cells was performed using multicolor flow cytometry.
(a) In the absence of acute or chronic chorioamnionitis, total B cells were more abundant in the decidua parietalis of women who delivered preterm than in those who delivered at term, regardless of the presence of labor; (b) decidual transitional and naïve B cells were the most abundant B-cell subsets; (c) decidual B1 B cells were increased in women with either labor at term or preterm labor and chronic chorioamnionitis compared to those without this placental lesion; (d) decidual transitional B cells were reduced in women with preterm labor compared to those without labor; (e) naïve, class-switched, and non-class-switched B cells in the decidual tissues underwent mild alterations with the process of preterm labor; (f) decidual plasmablasts seemed to increase in women with either labor at term or preterm labor with chronic chorioamnionitis; and (g) decidual B cells expressed high levels of interleukin (IL)-12, IL-6, and/or IL-35.
Total B cells are not increased with the presence of preterm or term labor; yet, specific subsets (B1 and plasmablasts) undergo alterations in women with chronic chorioamnionitis. Therefore, B cells are solely implicated in the pathological process of preterm labor in a subset of women with chronic inflammation of the placenta. These findings provide insight into the immunology of the maternal-fetal interface in preterm and term labor.
足月和早产临产时母体-胎儿界面（蜕膜）中 B 细胞的免疫表型尚未完全清楚。
从早产或足月临产以及非临产、同期的年龄匹配对照组的妇女中获取蜕膜组织。使用多色流式细胞术对蜕膜 B 细胞进行免疫表型分析。
（a）在没有急性或慢性绒毛膜羊膜炎的情况下，与足月临产的妇女相比，早产临产的妇女的蜕膜中总 B 细胞更为丰富，而与是否临产无关；（b）蜕膜中转和幼稚 B 细胞是最丰富的 B 细胞亚群；（c）与无胎盘病变的妇女相比，无论足月临产还是早产临产伴慢性绒毛膜羊膜炎的妇女，蜕膜 B1 B 细胞均增加；（d）与无临产的妇女相比，早产临产的妇女的蜕膜中转态 B 细胞减少；（e）与无临产的妇女相比，早产临产妇女的蜕膜幼稚、类别转换和非类别转换 B 细胞发生轻度改变；（f）蜕膜浆母细胞似乎在伴慢性绒毛膜羊膜炎的足月或早产临产妇女中增加；（g）蜕膜 B 细胞表达高水平的白细胞介素（IL）-12、IL-6 和/或 IL-35。
总 B 细胞的数量不会因早产或足月临产而增加；然而，在患有慢性绒毛膜羊膜炎的妇女中，特定亚群（B1 和浆母细胞）发生改变。因此，B 细胞仅参与部分患有胎盘慢性炎症妇女的早产临产的病理过程。这些发现为足月和早产临产时母体-胎儿界面的免疫学提供了新的认识。
