Hartung Nicole M, Mainka Malwina, Kampschulte Nadja, Ostermann Annika I, Schebb Nils Helge
Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences, University of Wuppertal, Gaußstraße 20, 42119 Wuppertal, Germany.
Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences, University of Wuppertal, Gaußstraße 20, 42119 Wuppertal, Germany.
Prostaglandins Other Lipid Mediat. 2019 Apr;141:22-24. doi: 10.1016/j.prostaglandins.2019.02.006. Epub 2019 Feb 12.
Quantitative analysis of oxylipins by means of chromatography/mass spectrometry is based on (external) calibration with standard compounds. Therefore, the quality of analytical standards is of fundamental importance for accurate results. Recently launched certified standards with an assured concentration within a narrow range are useful tools to verify analytical standards. However, such standards are only available for a few compounds. Based on the exemplary comparison of certified with none certified standards we suggest a tiered approach to validate and control the concentrations when preparing an external calibration based on non-certified oxylipin standards. Concentrations are evaluated by means of liquid chromatography negative electrospray ionization mass spectrometry (LC-ESI(-)-MS) in selected ion monitoring mode and UV spectroscopy. Based on the suggested approach, more than 50% of the standards in our calibration mix could be validated. Though most of the non-certified standards are of good quality, several oxylipin concentrations differ considerably demonstrating that a quality control strategy as suggested here is a mandatory prerequisite for quantitative oxylipin metabolomics.
通过色谱/质谱法对氧化脂质进行定量分析是基于用标准化合物进行（外部）校准。因此，分析标准品的质量对于获得准确结果至关重要。最近推出的在狭窄范围内具有确定浓度的认证标准品是验证分析标准品的有用工具。然而，此类标准品仅适用于少数化合物。基于认证标准品与非认证标准品的示例比较，我们建议采用分级方法来验证和控制基于非认证氧化脂质标准品进行外部校准时的浓度。通过液相色谱负电喷雾电离质谱（LC-ESI(-)-MS）在选择离子监测模式下以及紫外光谱法对浓度进行评估。基于所建议的方法，我们校准混合物中超过50%的标准品可以得到验证。尽管大多数非认证标准品质量良好，但几种氧化脂质的浓度差异很大，这表明此处建议的质量控制策略是定量氧化脂质代谢组学的强制性先决条件。
