Martínez-Zubiaur Y, Fiallo-Olivé E, Carrillo-Tripp J, Rivera-Bustamante R
Dirección Protección de Plantas, CENSA, San José de las Lajas, Habana, Cuba.
Facultad Biología, Universidad La Habana, Cuba.
Plant Dis. 2008 May;92(5):836. doi: 10.1094/PDIS-92-5-0836C.
Whitefly-transmitted viruses have caused severe losses in tomato crops (Solanum lycopersicum) in Cuba. In 2006 and 2007, tomato greenhouses across eastern Cuba exhibited high levels of Bemisia tabaci (B biotype) infestation. Some plants showed interveinal chlorosis and a severe yellow mosaic, combined with leaf brittleness. These symptoms were different from those induced by Tomato yellow leaf curl virus (TYLCV-IL(CU)). Only 12 of 31 symptomatic samples resulted in positive PCR assays with TYLCV-specific primers (CTGAATGTTTGGATGGAAATGTGC and GCTCGTAAGTTTCCTCAACGGAC). A reverse transcription (RT)-PCR analysis for Tomato chlorosis virus (ToCV) with generic (HS-11/HS-12) and specific primers (ToC-5/ToC-6) was also carried out (2). Sequence analysis of the cloned RT-PCR products (463 bp) confirmed the presence of ToCV in Cuba. The fragment had 97 to 98% identity with GenBank isolates from Spain (DQ136146), Florida (AY903448), and Reunion Island, France (AJ968396). Cloned TYLCV and ToCV amplicons were used as probes to reanalyze the selected 31 samples by a dot-blot hybridization assay in search of mixed infections (1). The assay showed 16 samples to be positive for ToCV, 4 for TYLCV, 8 for both, and 3 samples were negative. To our knowledge, this is the first report of ToCV and TYLCV/ToCV mixed infections in Cuba. References: (1) Y. Abou-Jawdha et al. Plant Dis. 90:378, 2006. (2) C. I. Dovas et al. Plant Dis. 86:1345, 2002.
粉虱传播的病毒已在古巴的番茄作物（番茄）中造成严重损失。2006年和2007年，古巴东部的番茄温室中烟粉虱（B生物型）的侵染水平很高。一些植株出现脉间黄化和严重的黄色花叶病，并伴有叶片脆化。这些症状与番茄黄化曲叶病毒（TYLCV-IL(CU)）引起的症状不同。在31个有症状的样本中，只有12个用TYLCV特异性引物（CTGAATGTTTGGATGGAAATGTGC和GCTCGTAAGTTTCCTCAACGGAC）进行PCR检测呈阳性。还使用通用引物（HS-11/HS-12）和特异性引物（ToC-5/ToC-6）对番茄褪绿病毒（ToCV）进行了逆转录（RT）-PCR分析（2）。对克隆的RT-PCR产物（463 bp）进行的序列分析证实古巴存在ToCV。该片段与来自西班牙（DQ136146）、佛罗里达（AY903448）和法国留尼汪岛（AJ968396）的GenBank分离株具有97%至98%的同一性。克隆的TYLCV和ToCV扩增子用作探针，通过斑点杂交试验对选定的31个样本进行重新分析，以寻找混合感染情况（1）。试验显示，16个样本ToCV呈阳性，4个样本TYLCV呈阳性，8个样本两者均呈阳性，3个样本呈阴性。据我们所知，这是古巴首次报道ToCV和TYLCV/ToCV混合感染。参考文献：（1）Y. Abou-Jawdha等人，《植物病害》90:378，2006年。（2）C. I. Dovas等人，《植物病害》86:1345，2002年。
