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基于蛋白质组学分析鉴定氯化锌烟雾吸入致伤肺中的差异表达蛋白。

Identification of differentially expressed proteins in the injured lung from zinc chloride smoke inhalation based on proteomics analysis.

机构信息

Medical School of Chinese PLA, Medical School of Chinese PLA, Fuxing Road, Beijing, 100853, China.

Department of Respiratory, The Fourth Medical Center of Chinese PLA General Hospital, Beijing, China.

出版信息

Respir Res. 2019 Feb 15;20(1):36. doi: 10.1186/s12931-019-0995-0.

DOI:10.1186/s12931-019-0995-0
PMID:30770755
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6377712/
Abstract

BACKGROUND

Lung injury due to zinc chloride smoke inhalation is very common in military personnel and leads to a high incidence of pulmonary complications and mortality. The aim of this study was to uncover the underlying mechanisms of lung injury due to zinc chloride smoke inhalation using a rat model.

METHODS

Histopathology analysis of rat lungs after zinc chloride smoke inhalation was performed by using haematoxylin and eosin (H&E) and Mallory staining. A lung injury rat model of zinc chloride smoke inhalation (smoke inhalation for 1, 2, 7 and 14 days) was developed. First, isobaric tags for relative and absolute quantization (iTRAQ) and weighted gene co-expression network analysis (WGCNA) were used to identify important differentially expressed proteins. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to study the biological functions of differentially expressed proteins. Then, analysis of lung injury repair-related differentially expressed proteins in the early (day 1 and day 2) and middle-late stages (day 7 and day 14) of lung injury after smoke inhalation was performed, followed by the protein-protein interaction (PPI) analysis of these differentially expressed proteins. Finally, the injury repair-related proteins PARK7 and FABP5 were validated by immunohistochemistry and western blot analysis.

RESULTS

Morphological changes were observed in the lung tissues after zinc chloride smoke inhalation. A total of 27 common differentially expressed proteins were obtained on days 1, 2, 7 and 14 after smoke inhalation. WGCNA showed that the turquoise module (which involved 909 proteins) was most associated with smoke inhalation time. Myl3, Ckm, Adrm1 and Igfbp7 were identified in the early stages of lung injury repair. Gapdh, Acly, Tnni2, Acta1, Actn3, Pygm, Eno3 and Tpi1 (hub proteins in the PPI network) were identified in the middle-late stages of lung injury repair. Eno3 and Tpi1 were both involved in the glycolysis/gluconeogenesis signalling pathway. The expression of PARK7 and FABP5 was validated and was consistent with the proteomics analysis.

CONCLUSION

The identified hub proteins and their related signalling pathways may play crucial roles in lung injury repair due to zinc chloride smoke inhalation.

摘要

背景

锌烟雾吸入引起的肺损伤在军事人员中非常常见，导致肺部并发症和死亡率很高。本研究旨在使用大鼠模型揭示锌烟雾吸入引起的肺损伤的潜在机制。

方法

采用苏木精和伊红（H&E）和Mallory 染色对吸入锌烟雾后的大鼠肺组织进行组织病理学分析。建立了锌烟雾吸入性肺损伤大鼠模型（烟雾吸入 1、2、7 和 14 天）。首先，采用等压标签相对和绝对定量（iTRAQ）和加权基因共表达网络分析（WGCNA）鉴定重要差异表达蛋白。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）途径分析研究差异表达蛋白的生物学功能。然后，分析吸入烟雾后肺损伤早期（第 1 天和第 2 天）和中晚期（第 7 天和第 14 天）与肺损伤修复相关的差异表达蛋白，并对这些差异表达蛋白进行蛋白-蛋白相互作用（PPI）分析。最后，通过免疫组织化学和 Western blot 分析验证损伤修复相关蛋白 PARK7 和 FABP5。

结果

锌烟雾吸入后观察到肺组织的形态学变化。吸入烟雾后 1、2、7 和 14 天共获得 27 个共同差异表达蛋白。WGCNA 显示，绿松石模块（涉及 909 种蛋白）与烟雾吸入时间最相关。在肺损伤修复的早期阶段鉴定出 Myl3、Ckm、Adrm1 和 Igfbp7。在肺损伤修复的中晚期阶段鉴定出 Gapdh、Acly、Tnni2、Acta1、Actn3、Pygm、Eno3 和 Tpi1（PPI 网络中的枢纽蛋白）。Eno3 和 Tpi1 均参与糖酵解/糖异生信号通路。验证了 PARK7 和 FABP5 的表达，与蛋白质组学分析一致。

结论

鉴定出的枢纽蛋白及其相关信号通路可能在锌烟雾吸入引起的肺损伤修复中发挥关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/665b504402a2/12931_2019_995_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/9e977c5202b8/12931_2019_995_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/be3d15973f3b/12931_2019_995_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/9dca721011e0/12931_2019_995_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/ce3ec989efc4/12931_2019_995_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/b6fffe9dfd38/12931_2019_995_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/665b504402a2/12931_2019_995_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/9e977c5202b8/12931_2019_995_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/2cc2d18b76e0/12931_2019_995_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/1903151f4391/12931_2019_995_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/11724cdc006d/12931_2019_995_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/be3d15973f3b/12931_2019_995_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/9dca721011e0/12931_2019_995_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/ce3ec989efc4/12931_2019_995_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/b6fffe9dfd38/12931_2019_995_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/27b6/6377712/665b504402a2/12931_2019_995_Fig11_HTML.jpg

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2
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Genomics. 2019 Jan;111(1):76-89. doi: 10.1016/j.ygeno.2018.01.003. Epub 2018 Jan 6.
3
Identification of differentially expressed genes, lncRNAs and miRNAs which are associated with tumor malignant phenotypes in hepatoblastoma patients.
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Front Med (Lausanne). 2023 Jan 12;9:1030644. doi: 10.3389/fmed.2022.1030644. eCollection 2022.
4
Characterization of the circRNA-miRNA-mRNA Network to Reveal the Potential Functional ceRNAs Associated With Dynamic Changes in the Meat Quality of the Longissimus Thoracis Muscle in Tibetan Sheep at Different Growth Stages.环状RNA-微小RNA-信使RNA网络的特征分析，以揭示与不同生长阶段藏羊胸最长肌肉质动态变化相关的潜在功能性竞争性内源RNA
Front Vet Sci. 2022 Apr 1;9:803758. doi: 10.3389/fvets.2022.803758. eCollection 2022.
5
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6
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10
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Oncotarget. 2017 Oct 31;8(57):97554-97564. doi: 10.18632/oncotarget.22181. eCollection 2017 Nov 14.
4
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6
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7
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Medicine (Baltimore). 2017 Sep;96(39):e8156. doi: 10.1097/MD.0000000000008156.
8
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9
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Medicine (Baltimore). 2017 Aug;96(34):e7564. doi: 10.1097/MD.0000000000007564.