Department of Morphological and Health Sciences, Faculty of Physical Culture in Gorzów Wielkopolski, 13 Estkowskiego Str, 66-400, Gorzów Wielkopolski, Poland.
Department of Water Sports, Faculty of Physical Culture in Gorzów Wielkopolski, 13 Estkowskiego Str, 66-400, Gorzów Wielkopolski, Poland.
J Int Soc Sports Nutr. 2019 Feb 15;16(1):7. doi: 10.1186/s12970-019-0274-y.
The aim of this study was to analyze the response of selected components of the immune system in rowers to maximal physical exercise, and to verify if this response could be modulated by supplementation with L-theanine.
The double-blind study included 20 members of the Polish Rowing Team. The subjects were randomly assigned to the supplemented group (n = 10), receiving 150 mg of L-theanine extract for 6 weeks, or to the placebo group (n = 10). The participants performed a 2000-m test on a rowing ergometer at the beginning (1st examination) and at the end of the supplementation period (2nd examination). Blood samples were obtained from the antecubital vein before each exercise test, 1 min after completing the test, and after a 24-h recovery. Subpopulations of T regulatory lymphocytes (Tregs) (CD4+/CD25+/CD127-), cytotoxic lymphocytes (CTLs) (CD8+/TCRαβ+), natural killer (NK) cells (CD3-/CD16+/CD56+) and TCRδγ-positive (Tδγ) cells were determined by means of flow cytometry. The levels of interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 10 (IL-10), interferon gamma (INF-ɤ) and total antioxidant capacity (TAC) were determined with commercially available diagnostic kits.
Supplementation with L-theanine contributed to a significant post-exercise decrease in IL-10 concentration, which was reflected by higher values of IL-2 to IL-10 and IFN-γ to IL-10 ratios. Moreover, a significant post-recovery decrease in CTL count, Treg to NK and Treg to CTL ratios was observed in the supplemented group.
Despite the decrease in the number of some cytotoxic cells (CTLs) and an increase in the proportion of Tregs to CTLs, supplementation with LTE seems to exert a beneficial effect on a disrupted Th1/Th2 balance in elite athletes, as shown by the decrease in IL-10 concentration.
本研究旨在分析划船运动员在最大运动量下免疫系统的特定成分的反应，并验证补充 L-茶氨酸是否可以调节这种反应。
这项双盲研究纳入了 20 名波兰赛艇队队员。将受试者随机分为补充组（n=10），每天接受 150mg L-茶氨酸提取物补充 6 周，或安慰剂组（n=10）。在研究开始（第 1 次检查）和补充周期结束时（第 2 次检查），受试者均在划船测功仪上进行 2000m 测试。在每次运动测试前、测试后 1 分钟以及 24 小时恢复后，从前臂静脉采集血样。采用流式细胞术测定 T 调节性淋巴细胞（Tregs）（CD4+/CD25+/CD127-）、细胞毒性淋巴细胞（CTL）（CD8+/TCRαβ+）、自然杀伤（NK）细胞（CD3-/CD16+/CD56+）和 TCRδγ阳性（Tδγ）细胞亚群。采用商业试剂盒测定白细胞介素 2（IL-2）、白细胞介素 4（IL-4）、白细胞介素 10（IL-10）、干扰素 γ（INF-ɤ）和总抗氧化能力（TAC）水平。
补充 L-茶氨酸可显著降低运动后 IL-10 浓度，这表现为 IL-2 与 IL-10 以及 INF-γ 与 IL-10 的比值增加。此外，补充组在恢复后 CTL 计数、Treg 与 NK 以及 Treg 与 CTL 的比值显著降低。
尽管一些细胞毒性细胞（CTL）的数量减少，Treg 与 CTL 的比例增加，但补充 L-茶氨酸似乎对精英运动员中失调的 Th1/Th2 平衡产生有益影响，这表现为 IL-10 浓度降低。
