Koehler M, Bubała H, Janik-Moszant A
Pediatric and Haematology Clinic, Silesian School of Medicine, Zabrze.
Arch Immunol Ther Exp (Warsz). 1988;36(5):585-93.
To optimalize the diagnosis of meningeal involvement in ALL we adapted the alkaline-phosphatase-anti-alkaline-phosphatase procedure (APAAP) to be used for cerebrospinal fluid (CSF) examination. The study was performed on 19 patients (9 children with leukemia) with CSF hypercellularity. 490 determinations with a panel of 12 monoclonal antibodies were performed. This method was convenient, cell morphology was preserved so that combined morphological and immunological characterization of specific cells was possible in mixed cell populations. The combined study of immunological and morphological criteria produced more accurate results than those based on morphological criteria alone. The APAAP technique is useful for small numbers of cells. The preservation of antigenic reactivity in frozen CSF smears may be of use in reevaluation studies.
为优化急性淋巴细胞白血病（ALL）脑膜受累的诊断，我们采用碱性磷酸酶-抗碱性磷酸酶法（APAAP）用于脑脊液（CSF）检查。该研究对19例脑脊液细胞增多的患者（9例白血病儿童）进行。使用一组12种单克隆抗体进行了490次检测。该方法简便，细胞形态得以保留，因此在混合细胞群体中对特定细胞进行形态学和免疫学联合鉴定成为可能。免疫和形态学标准的联合研究比仅基于形态学标准产生更准确的结果。APAAP技术适用于少量细胞。冷冻脑脊液涂片抗原反应性的保留可能有助于重新评估研究。
