Veerman A J, Huismans L D, van Zantwijk I C
Leuk Res. 1985;9(9):1195-200. doi: 10.1016/0145-2126(85)90111-0.
The immunological phenotype of cells in the cerebrospinal fluid (CSF) was determined with immunoperoxidase techniques. A medium was used which allowed cells to stand at room temperature for 24 h without appreciable loss of cells. From 62 patients a total number of 208 CSF specimens were analysed. It proved possible to perform 4 determinations and a control reaction on 2.5 ml of CSF: cell count, cytology, E-rosetting and staining with a monoclonal antibody, provided that more than 1 cell per mm3 were present. This study focussed on the presence of the common acute lymphoblastic leukemia marker (cALL, determined with the monoclonal antibody J5). All 21 CSF specimens containing more than 5% cALL positive cells were from patients with an initial diagnosis of common ALL, 8 of these samples were considered to be normal and 3 uncertain by standard cytological criteria. Six of the 8 samples which were cytologically normal, were from patients who had clear meningeal involvement at diagnosis, or developed a relapse later. There were no patients who developed a meningeal relapse on cytological criteria that was not detected by immunocytology. In one patient a cytological diagnosis of meningeal relapse was not confirmed by immunocytology, this patient is disease free 2 yr later without cytostatic treatment. Immunoperoxidase methods to detect cALL positive cells in CSF are an invaluable aid to the diagnosis of meningeal leukemia.
采用免疫过氧化物酶技术测定脑脊液（CSF）中细胞的免疫表型。使用一种培养基，使细胞能在室温下放置24小时而细胞数量无明显损失。对62例患者的208份CSF标本进行了分析。结果表明，只要每立方毫米CSF中细胞数超过1个，就可以对2.5毫升CSF进行4项检测和1项对照反应：细胞计数、细胞学检查、E玫瑰花结形成试验以及用单克隆抗体染色。本研究重点关注常见急性淋巴细胞白血病标志物（cALL，用单克隆抗体J5测定）的存在情况。所有21份含有超过5% cALL阳性细胞的CSF标本均来自初诊为常见ALL的患者，其中8份样本根据标准细胞学标准被认为正常，3份不确定。8份细胞学正常的样本中，有6份来自诊断时存在明确脑膜受累或后来复发的患者。没有患者出现细胞学标准下未被免疫细胞学检测到的脑膜复发。在1例患者中，免疫细胞学未证实细胞学诊断的脑膜复发，该患者在未接受细胞抑制治疗的情况下2年后无病生存。免疫过氧化物酶法检测CSF中cALL阳性细胞对脑膜白血病的诊断具有重要价值。
