Hammer Anne, de Koning Maurits Nc, Blaakaer Jan, Steiniche Torben, Doorbar John, Griffin Heather, Mejlgaard Else, Svanholm Hans, Quint Wim Gv, Gravitt Patti E
Department of Obstetrics and Gynecology, Aarhus University Hospital, Denmark; Department of Clinical Medicine, Aarhus University, Denmark.
DDL Diagnostic Laboratory, Rijswijk, the Netherlands.
Papillomavirus Res. 2019 Jun;7:82-87. doi: 10.1016/j.pvr.2019.02.004. Epub 2019 Feb 14.
In this study, we aimed to provide molecular evidence of HPV latency in humans and discuss potential challenges of conducting studies on latency. We analyzed the entire cervix of two women who underwent hysterectomy unrelated to cervical abnormality. The cervices were sectioned into 242 and 186 sets respectively, and each set was tested separately for HPV using the SPF10-PCR-DEIA-LiPA25 system. To identify whether there was any evidence of transforming or productive infection, we used the biomarkers E4 and P16 to stain slides immediately adjacent to HPV-positive sections. HPV was detected in both cervices. In patient 1, 1/242 sets was positive for HPV31. In patient 2, 13/186 sets were positive for HPV18 and 1/186 was positive for HPV53. The infection was very focal in both patients, and there was no sign of a transforming or productive infection, as evaluated by the markers E4 and P16. Had we only analyzed one set from each block, the probability of detecting the infection would have been 32.3% and 2%, respectively.Our findings support the idea that HPV may be able to establish latency in the human cervix; however, the risk associated with a latent HPV infection remains unclear.
在本研究中，我们旨在提供人乳头瘤病毒（HPV）在人体内潜伏的分子证据，并讨论开展潜伏性研究的潜在挑战。我们分析了两名因与宫颈异常无关而行子宫切除术的女性的整个子宫颈。将子宫颈分别切成242组和186组，每组分别使用SPF10-PCR-DEIA-LiPA25系统进行HPV检测。为了确定是否有转化性或增殖性感染的证据，我们使用生物标志物E4和P16对紧邻HPV阳性切片的玻片进行染色。在两个子宫颈中均检测到HPV。在患者1中，242组中有1组HPV31呈阳性。在患者2中，186组中有13组HPV18呈阳性，186组中有1组HPV53呈阳性。两名患者的感染都非常局限，根据E4和P16标志物评估，没有转化性或增殖性感染的迹象。如果我们仅从每个组织块中分析一组，检测到感染的概率分别为32.3%和2%。我们的研究结果支持HPV可能能够在人类子宫颈中建立潜伏状态的观点；然而，潜伏性HPV感染相关的风险仍不清楚。
